Czech J. Genet. Plant Breed., 2006, 42(4):132-136 | DOI: 10.17221/3643-CJGPB

Estimation of Fatty Acid Content in Intact Seeds of Oilseed Rape (Brassica napus L.) Lines Using Near-Infrared Spectroscopy

Radoslav Koprna1, Pavel Nerušil3, Oldřich Kolovrat1, Vratislav Kučera2, Alois Kohoutek3
1 OSEVA PRO Ltd., dep. Research Institute of Oilseed Crops at Opava, Opava, Czech Republic
2 Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
3 Research Station of Grass Ecosystems at Jevíčko, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

Based on NIRS (near infrared reflectance spectroscopy) measurements carried out in a collection of 262 samples of winter oilseed rape with a different content of fatty acids (FA) in oil, calibration equations for the laboratory instrument Foss-NIRSystem 6500 were developed. Calibration was focused on the possibility of screening seed samples of different composition of oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3) using NIRS analysis. The reference method was gas chromatography (GC). The content of FA in segregating F2 generations after crossing between lines with different contents of FA and lines with standard content of FA in oil ranged from 32.3 to 82.0% for C18:1, 10.2-26.8% for C18:2 and 3.3-11.8% for C18:3. The verification of a validation equation in 50 randomly selected samples of F2 generation in the year 2006 proved high correlation coefficients (r) between NIRS analysis and GC values, r = 0.86 for C18:1, r = 0.82 for C18:2 and r = 0.85 for C18:3. Non-destructive NIRS analysis enables rapid and reliable selection of materials with different composition of FA in the seed of oilseed rape (lines with desirable high content of C18:1 and low content of C18:3).

Keywords: winter oilseed rape; fatty acids; oleic acid; linoleic acid; linolenic acid; NIRS calibration

Published: December 31, 2006  Show citation

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Koprna R, Nerušil P, Kolovrat O, Kučera V, Kohoutek A. Estimation of Fatty Acid Content in Intact Seeds of Oilseed Rape (Brassica napus L.) Lines Using Near-Infrared Spectroscopy. Czech J. Genet. Plant Breed. 2006;42(4):132-136. doi: 10.17221/3643-CJGPB.
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    References

    1. CARRÉ P., DARTENUC C., EVRARD J., JUDDE A., LABALETTE F., RAOUX E., RENARD M. (2003): Frying stability of rapeseed oils with modified fatty acid composition. In: Proc. 11th Int. Rapeseed Congr., Copenhagen, 540-543.
    2. JOURDREN C., SIMONNEAUX D., RENARD M. (1996): Selection on pollen for linolenic acid content in rapeseed Brassica napus L. Plant Breeding, 115: 11-15. Go to original source...
    3. KOLOVRAT O. (1985): Esterification of the fatty acids of rapeseed oil. Rostlinná výroba, 31: 777-782. (in Czech)
    4. LIU R.J., TANG Z.J. (1992): Study on quality characters in rapeseed germplasm resources. Journal of Southwest Agricultural University, 13: 537-542.
    5. MÍKA V., TILLMANN P., KOPRNA R., NERUŠIL P., KUČERA V. (2003): Fast prediction of quality parameters in whole seeds of oilseed rape (Brassica napus L.). Plant, Soil and Environment, 49: 141-145. Go to original source...
    6. PLEINES S., FRIEDT W. (1988): Breeding for improved C18 fatty acid composition in rapeseed (Brassica napus L.). Fat Science Technology, 90: 167-171 Go to original source...
    7. SATO T. (2002): New estimation method for fatty acid composition in oil using near infrared spectroscopy. Bioscience, Biotechnology and Biochemistry, 66: 2543-2548. Go to original source... Go to PubMed...
    8. SCARTH R., MCVETTY P. (1999): Designer oil canola a review of new food-grade Brassica oils with focus on high oleic, low linoleic types. In: Proc. 10th Int. Rapeseed Congr., Canberra-Australia. (CD-ROM)
    9. SCHIERHOLT A., BECKER H.C. (2001): Environmental variability and heritability of high oleic acid content in winter oilseed rape. Plant Breeding, 120: 63-66. Go to original source...
    10. SHENK J.S. (1990): Standardizing NIRS instruments. Proc. 3rd Int. Conf. Near Infrared Spectroscopy. Agriculture Research Centre Publication, Brussels, 649-654.
    11. SOMERS D.J., FRIESEN K.R.D., RAKOW G. (1998): Identification of molecular markers associated with linoleic acid desaturation in Brassica napus. Theoretical and Applied Genetics, 96: 897-903. Go to original source...
    12. VAN DE VOORT F.R., SEDMAN J., EMO G., ISMAIL A.A. (1992): Rapid and direct iodine value and saponification number determination on fats and oils by attenuated total reflectance. Fourier transform infrared spectroscopy. Journal of the American Oli Chemists' Society, 69: 1118-1121. Go to original source...
    13. VELASCO L., BECKER H.C. (1998): Estimating the fatty acid composition of the oil in intact-seed rapeseed (Brassica napus L.) by near-infrared spectroscopy. Euphytica, 101: 221-230. Go to original source...
    14. VELASCO L., GOFFMAN F.D., BECKER H.C. (1998): Variability for the fatty acid composition of the seed oil in a germplasm collection of the genus Brassica. Genetic Resources and Crop Evolution, 45: 371-382. Go to original source...
    15. VELASCO L., MÖLLERS C., BECKER H.C. (1999a): Estimation of seed weight, oil content and fatty acid composition in intact single seeds of rapeseed (Brassica napus L.) by near-infrared reflectance spectroscopy. Euphytica, 106: 79-85. Go to original source...
    16. VELASCO L., PERÉZ-VICH B., FERNÁNDEZ-MARTÍNEZ J.M. (1999b): Crop quality utilization. Crop Science, 39: 219-222. Go to original source...
    17. WILLIAMS P., SOBERING D. (1992): Whole-seed grain analysis by near-infrared transmittance and reflectance: a comparison. In: HILDRUM, K.I. et al. (eds.): Bridging the Gap between Data Analysis and NIR Application. Near Infra-red Spectroscopy. Ellis Horwood, Chicester, 441-446.

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