Czech J. Genet. Plant Breed., 2005, 41(2):73-78 | DOI: 10.17221/3674-CJGPB

Genetic Diversity between Yacon Landraces from Different Countries Based on Random Amplified Polymorphic DNAs

L Milella1, J Salava2, G Martelli1, I Greco1, E F Cusimamani3, I Viehmannová3
1 Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Universitá degli Studi della Basilicata, Potenza, Italy
2 Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
3 Institute of Tropical and Subtropical Agriculture, Czech University of Agriculture in Prague, Prague, Czech Republic

Random amplified polymorphic DNA (RAPD) markers are widely used for evaluating the genetic relationship of crop germplasm. Five different landraces of yacon (Smallantus sonchifolius (Poepp. and Hendl.) H. Robinson; Asteraceae) collected in various countries and showing different morphological traits were investigated using a total of 61 decamer primers. A total of 282 RAPD markers were scored and 28.7% of them were polymorphic at least within landraces. RAPD markers generated by one primer (OBP14) discriminated between all landraces. Markers were used to calculate genetic similarity coefficient and to build a dendrogram representing the genetic relationship between analysed landraces. The results suggest that RAPD markers could be used as a reliable tool to perform fingerprinting studies in Smallantus sonchifolius genome. This is the first report on the use of RAPDs to evaluate genetic distance and to distinguish between different landraces in yacon.

Keywords: Smallantus sonchifolius; yacon; RAPDs; fingerprinting

Published: June 30, 2005  Show citation

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Milella L, Salava J, Martelli G, Greco I, Cusimamani EF, Viehmannová I. Genetic Diversity between Yacon Landraces from Different Countries Based on Random Amplified Polymorphic DNAs. Czech J. Genet. Plant Breed. 2005;41(2):73-78. doi: 10.17221/3674-CJGPB.
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    References

    1. A J., S A., G A., S S. (2001): Hypoglycaemic effect of the water extract of Smallantus sonchifolius (yacon) leaves in normal and diabetic rats. Journal of Ethnopharmacology, 74: 125-132. Go to original source... Go to PubMed...
    2. C D., O J.H. (1997): Pedigree and RAPD-based DNA analysis of commercial U.S. rice cultivars. Crop Science, 37: 1630-1635. Go to original source...
    3. C R., A G.R., O C. (2003): Identification of RAPDs associated with resistance to lethal yellowing of the coconut (Cocos nucifera L.) palm. HortScience, 98: 257-263. Go to original source...
    4. D T.M., Y H. (1997): A linkage map of the diploid strawberry, Fragaria vesca. Journal of Heredity, 88: 215-221. Go to original source...
    5. E L., B R., B W.V., A A., M P., C A., S R., M R. (1992): Application of RFLP analysis to genetic linkage mapping in peaches. Horticultural Science, 27: 160-163. Go to original source...
    6. G A., R J. (1997): Yacon. Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson. In: H M., H J. (eds): Andean roots and Tubers: Ahipa, Arracacha, Maca, Yacon. Promoting the conservation and use of underutilized and neglected crops. 21. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome, Italy, 199-242.
    7. H M., W N.F., M A.G., L D.M. (1994): Molecular linkage map for apple. Journal of Heredity, 85: 4-11. Go to PubMed...
    8. H M., N A., O Y., Y T. (2000): Effect of activated carbon powder treatment on clarification, decolourization, deodorization and fructooligosaccharides content of yacon juice. Nippon Shokuhin Kagaku Kogaku Kaishi, 47: 148-154. (in Japanese) Go to original source...
    9. I A., T S., K H., N Y., A M., K O., A T., H Y. (1995): Antifungal melanpolides from leaf extract of Smallantus sonchifolius. Phytochemistry, 39: 845-848. Go to original source...
    10. L W.F. (1994): Computing Genetic Similarity Coefficients from RAPD Data: the Effect of PCR Artefacts. PCR Methods and Applications, 4: 31-37. Go to original source... Go to PubMed...
    11. L F., H M., K O. (2003): Purification and identification of antimicrobial sesquiterpere lactones from yacon (Smallantus sonchifolius) leaves. Bioscience, Biotechnology, and Biochemistry, 67: 2154-2159. Go to original source... Go to PubMed...
    12. L F., L F., B J.M., O P. (1995): DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity in Citrus. HortScience, 30: 1063-1067. Go to original source...
    13. M G., S F., G I., S M.R., P P., L A. (1999): Strawberry cultivars genetic relationship using randomly amplified polymorphic DNA (RAPD) analysis. Advances in Pharmaceutical Sciences, 13: 99-104.
    14. M A.E., M M.M., L M., W W.L., L K.R. (1991): Diversity and relationships among U.S. maize inbreds revealed by restriction fragment length polymorphisms. Crop Science, 31: 669-678. Go to original source...
    15. M T., O F., C A., T A.M., C J.I. (1996): Using RAPDs to study phylogenetic relationship in Rosa. Theoretical and Applied Genetics, 92: 273-277. Go to original source... Go to PubMed...
    16. N M. (1972): Genetic distance between populations. The American Naturalist, 106: 283-292. Go to original source...
    17. N M. (1978): Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583-590. Go to original source... Go to PubMed...
    18. O T., I O., Y S., I T., M K., K M., T T., A T. (1990): Composition of storage carbohydrate in tubers of yacon (Polimnia sonchifolia). Soil Science and Plant Nutrition, 36: 167-171. Go to original source...
    19. S B., V I., A ș S., V K., U J. (2003): Investigation of phenolic acids in yacon (Smallantus sonchifolius) leaves and tubers. Journal of Chromatography A, 1015: 89-98. Go to original source... Go to PubMed...
    20. W B.S. (1990): Genetic data analysis: methods for discrete population analysis. Sinauer, Sunderland, MA: 44-50. Recived for publication February 21, 2005

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