Czech Journal of Genetics and Plant Breeding, 2018 (vol. 54), issue 1
List of Reviewers 2017
editors
Czech J. Genet. Plant Breed., 2018, 54(1):I | DOI: 10.17221/10883-CJGPB 
Molecular identification of wheat leaf rust resistance genes in sixty Chinese wheat cultivarsOriginal Paper
Zhikuan REN, Zaifeng LI, Lingzhi SHI, Xiaodong WANG, Lin ZHU, Xing LI, Daqun LIU
Czech J. Genet. Plant Breed., 2018, 54(1):1-8 | DOI: 10.17221/6/2016-CJGPB
Common wheat (Triticum aestivum L.) is the major crop cultivated in Xinjiang and Anhui provinces of China. The climate in these two provinces is favourable for wheat leaf rust (Puccinia triticina) (Pt) infection. Here, we demonstrate a detailed investigation on the leaf rust resistance of 60 major wheat cultivars cultivated in these two regions. A mixture of high virulent Pt races (THTT, THTS, THTQ and PHPS) were used to phenotype all the collected wheat cultivars at an adult plant stage. Phenotypic disease severity (FDS) and the area under the disease progress curve (AUDPC) for each of these wheat cultivars were...
Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.)Original Paper
Ertan Sait KURTAR, Ahmet BALKAYA, Mehtap OZBAKIR OZER
Czech J. Genet. Plant Breed., 2018, 54(1):9-16 | DOI: 10.17221/30/2017-CJGPB
Although haploids were successfully produced via irradiated pollen technique and anther culture in Cucurbita maxima and Cucurbita moschata, the haploidization efficiency is still low due to genotype dependence. Thus, as an alternative technique, the efficacy of the ovule culture was investigated. Ovules were extracted at different flowering time and then cultured on a solid MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP), thidiazuron (TDZ), and naphthaleneacetic acid (NAA) to induce callogenesis and plant regeneration. The gynogenic response was influenced by the combination...
Isolation and characterization of polymorphic microsatellite markers in Toxicodendron vernicifluumOriginal Paper
Dinh-Duy VU, Thi Tuyet-Xuan BUI, Thi Hong-Nhung NGUYEN, Syed Noor Muhammad SHAH, Ngoc-Ha VU, Ya-Hong ZHU, Lei ZHANG, Yi ZHANG, Xiao-Hua HUANG
Czech J. Genet. Plant Breed., 2018, 54(1):17-25 | DOI: 10.17221/183/2016-CJGPB
A total 20 074 230 sequencing reads were generated by Illumina HiSeq™ 2500 from three different Toxicodendron vernicifluum tissue samples. In total, 48 693 unigenes with an average length of 703.34 bp were obtained by de novo assembly. 3392 potential EST-SSRs (expressed sequence tag-simple sequence repeat) were identified as potential molecular markers from unigenes with lengths exceeding 1 kb. A total of 80 pairs of PCR primers were randomly selected to validate the assembly quality and develop EST-SSR markers from genomic DNA. Of these primer pairs, 14 primer pairs successfully amplified DNA fragments and detected...
Lignin biosynthesis regulated by the antisense 4CL gene in alfalfaShort Communication
Jing MENG, Caona LI, Manlin ZHAO, Cheng WANG, Yi RU, Zhixin CUI, Yang HAN
Czech J. Genet. Plant Breed., 2018, 54(1):26-29 | DOI: 10.17221/23/2017-CJGPB
The Antisense 4CL gene was transfected into alfalfa through Agrobacterium-mediated transfer. The test results indicated that the antisense 4CL gene was successfully integrated into the genome DNA of alfalfa and was stably transmitted to the offspring. Compared to the wild-type plants, the lignin content of T0 and T1 generation plants was reduced by 45.77% and 31.97%, respectively; there were no significant differences in height and weight of T0 and T
Multiple DNA markers for evaluation of resistance against Potato virus Y, Potato virus S and Potato leafroll virusShort Communication
Masoud NADERPOUR, Leila SADEGHI
Czech J. Genet. Plant Breed., 2018, 54(1):30-33 | DOI: 10.17221/180/2016-CJGPB
Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified...
New late ripening apricot genotypes from a multipurpose apricot breeding programme in TurkeyShort Communication
Joanna Marie CROSS, Firat Ege KARAAT, Feyza İNCEOĞLU, Zehra Tuğba MURATHAN, Bayram Murat ASMA
Czech J. Genet. Plant Breed., 2018, 54(1):34-38 | DOI: 10.17221/159/2016-CJGPB
The harvest season of apricots is short and fruit cannot be protected in cold stores for a long time. For those reasons, apricot production and consumption are lower than in other fruit species. This study was conducted for the purpose of breeding new late ripening apricot varieties. To this aim, a total of 3718 hybrid genotypes of apricots were obtained from 42 crosses. Phenological, pomological and yield analyses were performed and variations of fruit development period, fruit size, polarimetric dry matter (Brix), total acidity, yield and other traits were determined. Two years of results for important traits are given for 12 promising genotypes....
List of field crop varieties registered in the Czech Republic in 2017New Varieties
Tomáš Mezlík
Czech J. Genet. Plant Breed., 2018, 54(1):39-40 | DOI: 10.17221/17/2018-CJGPB