Czech Journal of Genetics and Plant Breeding, 2005 (vol. 41), issue 4

Index of Volume 41Index

editors

Czech J. Genet. Plant Breed., 2005, 41(4):96-104 | DOI: 10.17221/6087-CJGPB

Theoretical Bases and Sources for Breeding Wheat for Combined Disease Resistance

V Šíp, P Bartoš, J Chrpová, A Hanzalová, L Širlová, J Šárová, V Dumalasová, L Čejka, A Hanišová, L Bobková, I Bížová, P Horčička

Czech J. Genet. Plant Breed., 2005, 41(4):127-143 | DOI: 10.17221/3659-CJGPB

Achievements and prospects of wheat breeding for disease resistance in the world and in theCzech Republic are discussed. Attention was paid to possibilities of increasing resistance to rusts, powdery mildew, Fusarium head blight, leaf blotch, glume blotch, tan spot, common bunt and barley yellow dwarf virus on wheat. Methodical approaches adopted in national ring infection tests were outlined. New sources of resistance to the above-mentioned diseases were detected and described on the basis of three-year results of field infection tests.

The Barley Microarray. A Community Vision and Application to Abiotic Stress

T J. Close

Czech J. Genet. Plant Breed., 2005, 41(4):144-152 | DOI: 10.17221/3660-CJGPB

A microarray chip representing approximately 20 000 barley unigenes was produced as part of a USA project entitled "An integrated physical and expression map of barley for Triticeae improvement". The content of the chip was derived from more than 400 000 barley "Expressed Sequence Tag" (EST) sequences received from cooperators inUSA,Germany,Australia,Japan,Scotland, andFinland, plus about 1000 sequences retrieved from the GenBank nr database or GrainGenes. All EST sequences were trimmed to high quality regions, contaminants were identified and removed, and the remaining information was compiled using the CAP3 assembly program. A "stringent"...

Genetic and Physical Mapping of Genic Microsatellites in Barley (Hordeum vulgare L.)

R K Varshney, U Hähnel, T Thiel, N Stein, L Altschmied, P Langridge, A Graner

Czech J. Genet. Plant Breed., 2005, 41(4):153-159 | DOI: 10.17221/3661-CJGPB

Due to the availability of sequence data from large-scale EST (expressed sequence tag) projects, it has become feasible to develop microsatellite or simple sequence repeat (SSR) markers from genes. A set of 111 090 barley ESTs (corresponding to 55.9 Mb of sequence) was employed for the identification of microsatellites with the help of a PERL5 script called MISA. As a result, a total of 9 564 microsatellites were identified in 8 766 ESTs (SSR-ESTs). Cluster analysis revealed the presence of 2 823 non-redundant SSR-ESTs in this set. From these 754 primer pairs were designed and analysed in a set of seven genotypes including the parents of three...

Powdery Mildew Resistance of Czech and Slovak Spring Barley Breeding Lines in Variety Trials

A Dreiseitl

Czech J. Genet. Plant Breed., 2005, 41(4):160-166 | DOI: 10.17221/3662-CJGPB

In 2001-2005, resistance to powdery mildew was studied in 227 Czech and Slovak breeding lines of spring barley included in the breeding station trials or official trials. Seventeen known resistances were identified (Al, Ar, At, HH, Kr, La, Ly, Mlo, N81, Ri, Ru, Sp, St, Tu, We, Mla21, and Mlp1). Unknown resistances were found in 11 breeding lines, in five of which resistance was effective against all used pathotypes of the pathogen. Besides the identified resistances, unknown resistances were detected in another three breeding lines. Sixty-five breeding lines (= 29%) exhibited heterogeneity in the examined trait, i.e. they are composed...

Oligogenic Inheritance of Resistance to Plum Pox Virus in Apricots

J Salava, J Polák, B Krška

Czech J. Genet. Plant Breed., 2005, 41(4):167-170 | DOI: 10.17221/3663-CJGPB

In order to determine the inheritance of resistance to PPV in apricot three crosses between resistant and susceptible cultivars and selections were performed. The B₁ seedlings were inoculated with the PPV-M strain by an infected bud. PPV infection was evaluated over 5 consecutive growth periods through visual symptoms, ELISA and in some cases reverse transcriptase PCR assays. Chi-square analysis of each B₁ progeny was performed to determine if the segregation ratio differed from the expected ratio. PPV resistance segregated in three apricot B₁progenies in a 1:7 (resistant:susceptible) ratio, indicating that resistance...