Czech J. Genet. Plant Breed., 2025, 61(1):31-42 | DOI: 10.17221/104/2024-CJGPB

High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2 Original Paper

Jan Dvorak1*, Karin R. Deal1, Patrick E. McGuire1, Emily J. Conley2, James A. Anderson2, George Fedak3, Julia A. Malvick4, Han Chen5, Hans-Georg Müller5
1 Department of Plant Sciences, University of California, Davis, USA
2 Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, USA
3 Ottawa Research and Development Center, Agriculture and Agri-Food Canada, Ottawa, Canada
4 Veterinary Genetics Laboratory, University of California, Davis, USA
5 Department of Statistics, University of California, Davis, USA

Wheat Fusarium head blight (FHB) leads to losses of grain yield and quality. Ingestion of diseased grain is detrimental to human health due to the mycotoxins present in the grain. Developing resistant cultivars for environments where FHB is prevalent is therefore an important breeding objective. One of the most effective wheat genes conferring type II resistance to FHB is Fhb1, originally discovered in the Chinese cultivar (cv) Sumai 3. Another excellent FHB resistance gene is Fhb7 located on the long arm of Lophopyrum elongatum chromosome 7E. Several alleles of Fhb7 have been identified. Allele Fhb7The2 was found in disomic substitution lines 7E(7A), 7E(7B) and 7E(7D) derived from amphiploid AgCS. The amphiploid was produced from a hybrid Triticum aestivum cv Chinese Spring × L. elongatum. To find if combining Fhb7The2 with Fhb1 confers higher resistance in wheat than single genes, an introgression line derived from AgCS and possessing Fhb7The2 was recurrently backcrossed to bread wheat cv Rollag and MN-Washburn possessing Fhb1. Experimental lines possessing both Fhb7The2 and Fhb1 were developed and validated cytogenetically and with the L. elongatum genome-wide Sequenom SNP MassARRAY. Spikes of these lines, parental cv Rollag and MN-Washburn, and those of disomic addition line 7E possessing Fhb7The2 plus controls were inoculated with Fusarium in a twice-replicated trial in controlled greenhouse environmental conditions. FHB infection rates were significantly lower in lines combining Fhb7The2 with Fhb1 than in materials with Fhb7The2 or Fhb1 alone.

Keywords: aneuploidy; bread wheat; disomic addition; ditelosomic addition; introgression; meiotic stability; Robertsonian translocation; Sequenom; SNP; Thinopyrum

Received: August 29, 2024; Revised: November 20, 2024; Accepted: November 25, 2024; Prepublished online: December 16, 2024; Published: January 14, 2025  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Dvorak J, Deal KR, McGuire PE, Conley EJ, Anderson JA, Fedak G, et al.. High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2 . Czech J. Genet. Plant Breed. 2025;61(1):31-42. doi: 10.17221/104/2024-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files:

    Download file104-2024_CJGPB_ESM_Table_S1.xlsx

    File size: 13.56 kB

    References

    1. Alisaac E., Mahlein A.K. (2023): Fusarium head blight on wheat: Biology, modern detection and diagnosis and integrated disease management. Toxins, 15: 192. Go to original source... Go to PubMed...
    2. Anderson J.A., Stack R.W., Liu S., Waldron B.L., Fjeld A.D., Coyne C., Moreno-Sevilla B., Fetch J.M., Song Q.J., Cregan P.B., Frohberg R.C. (2001): DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theoretical and Applied Genetics, 102: 1164-1168. Go to original source...
    3. Anderson J.A., Wiersma J.J., Linkert G.L., Reynolds S., Kolmer J.A., Jin Y., Dill-Macky R., Hareland G.A. (2015): Registration of 'Rollag' Spring Wheat. Journal of Plant Registrations, 9: 201-207. Go to original source...
    4. Anderson J.A., Wiersma J.J., Reynolds S.K., Conley E.J., Caspers R., Linkert G.L., Kolmer J.A., Jin Y., Rouse M.N., Dill-Macky R., Smithy M.J., Dykes L., Ohm J.B. (2021): Registration of 'MN-Washburn' hard red spring wheat containing resistance gene. Journal of Plant Registrations, 15: 490-503. Go to original source...
    5. Barkworth M.E. (1992): Taxonomy of the Triticeae: A historical perspective. Hereditas, 116: 1-14. Go to original source...
    6. Cai X.W., Danilova T., Charif A., Wang F., Zhang W., Zhang M.Y., Ren S.F., Zhu X.W., Zhong S.B., Dykes L., Fiedler J., Xu S., Frels K., Wegulo S., Boehm J., Funnell-Harris D. (2024): Registration of WGC002 spring wheat containing wild grass-derived Fusarium head blight resistance gene. Journal of Plant Registrations, 18: 179-186. Go to original source...
    7. Ceoloni C., Forte P., Kuzmanovic L., Tundo S., Moscetti I., De Vita P., Virili M.E., D'Ovidio R. (2017): Cytogenetic mapping of a major locus for resistance to Fusarium head blight and crown rot of wheat on Thinopyrum elongatum 7EL and its pyramiding with valuable genes from a Th. ponticum homoeologous arm onto bread wheat 7DL. Theoretical and Applied Genetics, 130: 2005-2024. Go to original source... Go to PubMed...
    8. Cuthbert P.A., Somers D.J., Thomas J., Cloutier S., Brule-Babel A. (2006): Fine mapping Fhb1, a major gene controlling fusarium head blight resistance in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 112: 1465-1472. Go to original source... Go to PubMed...
    9. Dvorak J. (1971): Reducing breakage of metaphase plates in Feulgen stained root tips of wheat; ice water storage before squashing. Stain Technology, 46: 93-94. Go to original source... Go to PubMed...
    10. Dvorak J. (1980): Homoeology between Agropyron elongatum chromosomes and Triticum aestivum chromosomes. Canadian Journal of Genetics and Cytology, 22: 237-259. Go to original source...
    11. Dvorak J., Chen K.C. (1984): Phylogenetic relationships between chromosomes of wheat and chromosome 2E of Elytrigia elongata. Canadian Journal of Genetics and Cytology, 26: 128-132. Go to original source...
    12. Dvorak J., Akhunov E.D., Akhunov A.R., Deal K.R., Luo M.C. (2006): Molecular characterization of a diagnostic DNA marker for domesticated tetraploid wheat provides evidence for gene flow from wild tetraploid wheat to hexaploid wheat. Molecular Biology and Evolution, 23: 1386-1396. Go to original source... Go to PubMed...
    13. Forte P., Virili M.E., Kuzmanovic L., Moscetti I., Gennaro A., D'Ovidio R., Ceoloni C. (2014): A novel assembly of Thinopyrum ponticum genes into the durum wheat genome: pyramiding Fusarium head blight resistance onto recombinant lines previously engineered for other beneficial traits from the same alien species. Molecular Breeding, 34: 1701-1716. Go to original source...
    14. Fu S.L., Lv Z.L., Qi B., Guo X., Li J., Liu B., Han F.P. (2012): Molecular cytogenetic characterization of wheat-Thinopyrum elongatum addition, substitution and translocation lines with a novel source of resistance to wheat Fusarium head blight. Journal of Genetics and Genomics, 39: 103-110. Go to original source... Go to PubMed...
    15. Giorgi B. (1978): A homoeologous pairing mutant isolated in Triticum durum cv. Cappelli. Mutation Breeding Newsletter, 11: 4-5.
    16. Grausgruber H., Lemmens M., Bürstmayr H., Ruckenbauer P. (1999): Resistance of 'Chinese Spring' substitution lines carrying chromosomes from 'Cheyenne', 'Hope' and 'Lutescens 62' wheats to head blight caused by Fusarium culmorum. Hereditas, 130: 57-63. Go to original source...
    17. Guo J., Zhang X.L., Hou Y.L., Cai J.J., Shen X.R., Zhou T.T., Xu H.H., Ohm H.W., Wang H.W., Li A.F., Han F.P., Wang H.G., Kong L.R. (2015): High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theoretical and Applied Genetics, 128: 2301-2316. Go to original source... Go to PubMed...
    18. Guo X.R., Wang M., Kang H.Y., Zhou Y.H., Han F.P. (2022): Distribution, polymorphism and function characteristics of the GST-encoding Fhb7 in Triticeae. Plants-Basel, 11: 2074. Go to original source... Go to PubMed...
    19. Haldar A., Tekieh F., Balcerzak M., Wolfe D., Lim D., Joustra K., Konkin D., Han F.P., Fedak G., Ouellet T. (2021): Introgression of Thinopyrum elongatum DNA fragments carrying resistance to fusarium head blight into Triticum aestivum cultivar Chinese Spring is associated with alteration of gene expression. Genome, 64: 1009-1020. Go to original source... Go to PubMed...
    20. Hart G.E., Tuleen N.A. (1983): Chromosomal control of eleven Elytrigia elongata (=Agropyron elongatum) isozyme structural genes. Genetic Research, 41: 181-202. Go to original source...
    21. Hou B.Q., Wang D.W., Yan F.F., Cheng X.X., Xu Y.C., Xi X.P., Ge W.Y., Sun S.L., Su P.S., Zhao L.F., Lyu Z., Hao Y.C., Wang H.W., Kong L.R. (2024): Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family. Food Chemistry, 439: 138057. Go to original source... Go to PubMed...
    22. Jauhar P.P., Peterson T.S. (1998): Wild relatives of wheat as sources of Fusarium head blight. National FHB Forum: 77-79.
    23. Löve A. (1984): Conspectus of the Triticeae. Feddes Repertorium, 95: 425-521. Go to original source...
    24. Luo M.C., Gu Y.Q., Puiu D., Wang H., Twardziok S.O., Deal K.R., Huo N.X., Zhu T.T., Wang L., Wang Y., McGuire P.E., Liu S.Y., Long H., Ramasamy R.K., Rodriguez J.C., Van S.L., Yuan L.X., Wang Z.Z., Xia Z.Q., Xiao L.C., Anderson O.D., Ouyang S.H., Liang Y., Zimin A.V., Pertea G., Qi P., Bennetzen J.L.B., Dai X.T., Dawson M.W., Muller H.G., Kugler K., Rivarola-Duarte L., Spannagl M., Mayer K.F.X., Lu F.H., Bevan M.W., Leroy P., Li P.C., You F.M., Sun Q.X., Liu Z.Y., Lyons E., Wicker T., Salzberg S.L., Devos K.M., Dvorak J. (2017): Genome sequence of the progenitor of the wheat D genome Aegilops tauschii. Nature, 551: 498-502. Go to original source... Go to PubMed...
    25. Ma H.X., Bai G.H., Gill B.S., Hart L.P. (2006): Deletion of a chromosome arm altered wheat resistance to Fusarium head blight and deoxynivalenol accumulation in Chinese Spring. Plant Disease, 90: 1545-1549. Go to original source... Go to PubMed...
    26. Ma Z.Q., Xie Q., Li G.Q., Jia H.Y., Zhou J.Y., Kong Z.X., Li N., Yuan Y. (2020): Germplasms, genetics and genomics for better control of disastrous wheat Fusarium head blight. Theoretical and Applied Genetics, 133: 1541-1568. Go to original source... Go to PubMed...
    27. McCullagh P. (2019): Generalized Linear Models. 2nd Ed. Boca Raton, Chapman and Hall/CRC.
    28. Miller S.S., Watson E.M., Lazebnik J., Gulden S., Balcerzak M., Fedak G., Ouellet T. (2011): Characterization of an alien source of resistance to Fusarium head blight transferred to Chinese Spring wheat. Botany-Botanique, 89: 301-311. Go to original source...
    29. Rommel R., Jenkins B.C. (1959): Amphiploids in Triticeae produced at the University of Manitoba from March 1958 to December 1959. Wheat Information Service, 9-10: 23.
    30. Rozen S., Skaletsky H.J. (2000): Primer3 on the WWW for general users and for biologist programmers. In: Krawet S., Misener S., Totowa N.J. (eds.): Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press: 365-386. Go to original source...
    31. Schweiger W., Steiner B., Vautrin S., Nussbaumer T., Siegwart G., Zamini M., Jungreithmeier F., Gratl V., Lemmens M., Mayer K.F.X., Bérgès H., Adam G., Buerstmayr H. (2016): Suppressed recombination and unique candidate genes in the divergent haplotype encoding FHb1, a major Fusarium head blight resistance locus in wheat. Theoretical and Applied Genetics, 129: 1607-1623. Go to original source... Go to PubMed...
    32. Sears E.R. (1977): An induced mutant with homoeologous pairing in common wheat. Canadian Journal of Genetics and Cytology, 19: 585-593. Go to original source...
    33. Shen R., Kong L.R., Ohm H. (2004): Fusarium head blight resistance in hexaploid wheat (Triticum aestivum)-Lophopyrum genetic lines and tagging of the alien chromatin by PCR markers. Theoretical and Applied Genetics, 108: 808-813. Go to original source... Go to PubMed...
    34. Su Z.Q., Jin S.J., Zhang D.D., Bai G.H. (2018): Development and validation of diagnostic markers for FHB region, a major QTL for head blight resistance in wheat. Theoretical and Applied Genetics, 131: 2371-2380. Go to original source... Go to PubMed...
    35. Tuleen N.A., Hart G.E. (1988): Isolation and characterization of wheat - Elytrigia elongata chromosome 3E and 5E addition and substitution lines. Genome, 30: 519-524. Go to original source...
    36. Waldron B.L., Moreno-Sevilla B., Anderson J.A., Stack R.W., Frohberg R.C. (1999): RFLP mapping of QTL for fusarium head blight resistance in wheat. Crop Science, 39: 805-811. Go to original source...
    37. Wang H., Sun S., Ge W., Zhao L., Hou B., Wang K., Lyu Z., Chen L., Xu S., Guo J., Li M., Su P., Li X., Wang G., Bo C., Fang X., Zhuang W., Cheng X., Wu J., Dong L., Chen W., Li W., Xiao G., Zhao J., Hao Y., Xu Y., Gao Y., Liu W., Liu Y., Yin H., Li J., Li X., Zhao Y., Wang X., Ni F., Ma X., Li A., Xu S.S., Bai G., Nevo E., Gao C., Ohm H., Kong L. (2020): Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat. Science, 368: eaba5435. Go to original source...
    38. Wang L.S., Zhang Y., Zhang M.Q., Gong D.C., Mei Y.Z., Dai C.C. (2023): Engineered Phomopsis liquidambaris with Fhb1 and Fhb7 enhances resistance to Fusarium graminearum in wheat. Journal of Agricultural and Food Chemistry, 71: 1391-1404. Go to original source... Go to PubMed...
    39. Xu J.L., Wang L., Deal K.R., Zhu T.T., Ramasamy R.K., Luo M.C., Malvick J., You F.M., McGuire P.E., Dvorak J. (2020): Genome-wide introgression from a bread wheat × Lophopyrum elongatum amphiploid into wheat. Theoretical and Applied Genetics, 133: 1227-1241. Go to original source... Go to PubMed...
    40. Zhang M.Y., Zhang W., Zhu X.W., Sun Q., Yan C.H., Xu S.S., Fiedler J., Cai X.W. (2020): Dissection and physical mapping of wheat chromosome 7B by inducing meiotic recombination with its homoeologues in Aegilops speltoides and Thinopyrum elongatum. Theoretical and Applied Genetics, 133: 3455-3467. Go to original source... Go to PubMed...
    41. Zhang W., Cao Y.P., Zhang M.Y., Zhu X.W., Ren S.F., Long Y.M., Gyawali Y., Chao S.M., Xu S., Cai X.W. (2017): Meiotic homoeologous recombination-based alien gene introgression in the genomics era of wheat. Crop Science, 57: 1189-1198. Go to original source...
    42. Zhang W., Zhu X.W., Zhang M.Y., Chao S.M., Xu S., Cai X.W. (2018): Meiotic homoeologous recombination-based mapping of wheat chromosome 2B and its homoeologues in Aegilops speltoides and Thinopyrum elongatum. Theoretical and Applied Genetics, 131: 2381-2395. Go to original source... Go to PubMed...
    43. Zhang W., Danilova T., Zhang M.Y., Ren S.F., Zhu X.W., Zhang Q.J., Zhong S.B., Dykes L., Fiedler J., Xu S., Frels K., Wegulo S., Boehm J., Cai X.W. (2022): Cytogenetic and genomic characterization of a novel tall wheatgrass-derived Fhb7 allele integrated into wheat B genome. Theoretical and Applied Genetics, 135: 4409-4419. Go to original source... Go to PubMed...
    44. Zhang X.L., Shen X.R., Hao Y.F., Cai J.J., Ohm H.W., Kong L.R. (2011): A genetic map of Lophopyrum ponticum chromosome 7E, harboring resistance genes to Fusarium head blight and leaf rust. Theoretical and Applied Genetics, 122: 263-270. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content