Czech J. Genet. Plant Breed., 2023, 59(3):133-140 | DOI: 10.17221/111/2022-CJGPB

A preliminary study on the root-knot nematode resistance of a cherry plum cultivar Mirabolano 29COriginal Paper

Jia Liu1, Jin Zhu3, Hongwen Li1, Deyong Luo4, Jialian Xie5, Huajia Li6, Shuo Liu7, Yuping Zhang7, Lijuan Chen1, Xiaodou Xie4, Dong Wang1, Ke Li6, Meiying Yao2, Guowei Zhang1
1 Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, PR China
2 Panzhihua Academy of Agriculture and Forestry Sciences, Panzhihua, Sichuan, PR China
3 Sichuan Horticultural Crop Technology Promotion Station, Chengdu, P.R. China
4 Sichuan Fruit Tree Breeding Station, Sichuan, PR China
5 Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Key Laboratory of Integrated Management of Pests on Crops in Southwest China, Ministry of Agriculture and Rural Affairs, Chengdu, Sichuan, P.R. China
6 Institute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu, P.R. China
7 Liaoning Institute of Pomology, Yingkou, Liaoning, P.R. China

Grafting is the most important means of breeding peach, plum, apricot, and other fruit trees, and the selection of the rootstock is crucial to the quality of the grafting and the yield of the products. The traditional commonly used peach rootstock is susceptible to root-knot nematode infections, resulting in a decreased yield, while a variety of cherry plum rootstocks, Mirabolano 29C, is resistant to root-knot nematode. In this study, root-knot nematode infection experiments on seedlings of traditional peach rootstocks and Mirabolano 29C confirmed that Mirabolano 29C was indeed more resistant to root-knot nematodes. At the same time, we compared the roots of the root-knot nematode uninfected and infected Mirabolano 29C by transcriptome sequencing and found 3 176 differentially expressed genes. A further functional enrichment analysis of these genes found that the secondary metabolites, phenylpropane and flavonoids, may be responsible for the high resistance of Mirabolano 29C to root-knot nematodes. These results can provide a reference value for the disease resistance breeding of rootstocks.

Keywords: disease resistance; grafting; phenylpropane pathway; rootstocks; transcriptome sequencing

Received: December 14, 2022; Accepted: January 16, 2023; Prepublished online: March 13, 2023; Published: June 12, 2023  Show citation

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Liu J, Zhu J, Li H, Luo D, Xie J, Li H, et al.. A preliminary study on the root-knot nematode resistance of a cherry plum cultivar Mirabolano 29C. Czech J. Genet. Plant Breed. 2023;59(3):133-140. doi: 10.17221/111/2022-CJGPB.
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