Czech J. Genet. Plant Breed., 2022, 58(2):64-72 | DOI: 10.17221/69/2021-CJGPB

Morpho-molecular diversity study of rice cultivars in BangladeshOriginal Paper

Md Babul Akter ORCID...*,1, Azad Mosab-Bin2, Mohammad Kamruzzaman3, Reflinur Reflinur4, Nazmun Nahar5, Md Sohel Rana5, Md Imdadul Hoque3, Md Shahidul Islam2
1 Bangladesh Institute of Nuclear Agriculture (BINA), Sub-station, Satkhira, Bangladesh
2 Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh
3 Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh
4 Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, IAARD, Bogor, Indonesia
5 Bangladesh Institute of Nuclear Agriculture (BINA), Sub-station, Barishal, Bangladesh

Rice is one of the frontline cereals in the world and the major cultivated crop in Bangladesh. A total of eleven simple sequence repeats (SSRs) and thirteen sequence-tagged site (STS) markers were used to characterize twenty-four rice cultivars in Bangladesh. Twenty-four markers generated 60 alleles with 2.5 alleles per locus. The average polymorphism information content (PIC) value was 0.40, while the mean value of heterozygosity, gene diversity, and major allele frequency were recorded as 0.10, 0.48 and 0.62, respectively. However, the SSR markers showed more specificity and a higher discrimination power than the STS markers. The cluster analysis displayed four major clusters with a genetic similarity coefficient value of 0.73. The morphological analyses of the grain identified that Binadhan-20 and BRRI dhan34 had the longest and the shortest seed size, respectively, with a variable correlation between the seed length, width and length/width ratio. The phenol reaction test distinguished seven cultivars as japonica and seventeen cultivars as indica or an intermediate type. All these results regarding the phenotypic data and marker information will be useful for parental selection in modern rice breeding programmes.

Keywords: genetic diversity; phenol reaction test; rice cultivars; SSR marker; STS marker

Published: March 15, 2022  Show citation

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Akter MB, Mosab-Bin A, Kamruzzaman M, Reflinur R, Nahar N, Rana MS, et al.. Morpho-molecular diversity study of rice cultivars in Bangladesh. Czech J. Genet. Plant Breed. 2022;58(2):64-72. doi: 10.17221/69/2021-CJGPB.
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