Czech J. Genet. Plant Breed., 2020, 56(3):102-110 | DOI: 10.17221/96/2019-CJGPB

Grain hardness in triticale: a physical and molecular evaluationOriginal Paper

Veronika Kselíková ORCID...*,1, Tomáš Vyhnánek2, Pavel Hanáček2, Petr Martinek3
1 Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
2 Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic
3 Agrotest fyto, Ltd., Kroměříž, Czech Republic

Grain hardness is an important quality trait of cereals. In the present work, the particle size index (PSI) was used to assess the variability in grain hardness of thirteen triticale genotypes, three wheat genotypes and one rye genotype grown in two subsequent seasons. The PSI values of the triticale samples ranged between 7.7 to 19.2%, representing a medium hard to a very hard grain. Moreover, the sequencing of genes in the Hardness (Ha) locus revealed a limited genetic variability in the grain hardness genes, namely secaloindoline-a (Sina) and secaloindoline-b (Sinb). Two possible new allelic variants were identified, one for each of the secaloindoline genes. The existing variability in the grain hardness as well as polymorphisms in its candidate genes are a good starting point for efforts in breeding new varieties of triticale with improved grain hardness.

Keywords: friabilin; Ha locus; particle size index; puroindoline; secaloindoline

Published: September 30, 2020  Show citation

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Kselíková V, Vyhnánek T, Hanáček P, Martinek P. Grain hardness in triticale: a physical and molecular evaluation. Czech J. Genet. Plant Breed. 2020;56(3):102-110. doi: 10.17221/96/2019-CJGPB.
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