Czech J. Genet. Plant Breed., 2014, 50(4):268-277 | DOI: 10.17221/72/2014-CJGPB

The application of microsatellite analysis in barley malting quality breeding programmesOriginal Paper

Leona LEIŠOVÁ-SVOBODOVÁ1, Lenka TOMKOVÁ1, Tibor SEDLÁČEK2, Vratislav PSOTA3, Ladislav KUČERA1
1 Crop Research Institute, Prague-Ruzyně, Czech Republic
2 Selton, Stupice, Czech Republic
3 Research Institute of Brewing and Malting, Prague, Czech Republic

A set of 43 microsatellite loci was used to characterise the malting barley varieties and breeding lines used for the Protected Geographical Indication (PGI) "České pivo" ("Czech beer") and for other beer types. Genotype data were compared with technological malting quality parameters. The analysis of variance showed significant differences between varieties used for the given PGI and varieties used for other beer types in six variables. Three clusters of varieties specific to the PGI and three clusters specific to malting barley genotypes for other beer types were identified using a Bayesian approach. The remaining four clusters expressed effects of parent lines. The discrimination analysis based on malting quality and molecular variables identified only one barley breeding line suitable for the PGI "České pivo".

Keywords: "České pivo"; discriminant analysis; diversity; markers; SSR

Published: December 31, 2014  Show citation

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LEIŠOVÁ-SVOBODOVÁ L, TOMKOVÁ L, SEDLÁČEK T, PSOTA V, KUČERA L. The application of microsatellite analysis in barley malting quality breeding programmes. Czech J. Genet. Plant Breed. 2014;50(4):268-277. doi: 10.17221/72/2014-CJGPB.
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