Czech J. Genet. Plant Breed., 2013, 49(1):1-8 | DOI: 10.17221/64/2012-CJGPB

Detection of QTLs for important agronomical traits in hexaploid wheat using association analysisOriginal Paper

Ljiljana BRBAKLIC, Dragana TRKULJA, Ankica KONDIC-SPIKA, Sanja TRESKIC, Borislav KOBILJSKI
Small Grains Department, Institute of Field and Vegetable Crops, Novi Sad, Serbia

One of the main wheat breeder's goals is determining specific genomic regions which control important agronomical traits. Association analysis is a new strategy with high resolution in plant molecular breeding that could be used to improve the efficiency of marker assisted selection (MAS) for finding important QTLs (quantitative trait loci) or genes. A set of 96 diverse wheat genotypes was phenotypically measured during three growing seasons (2006/07, 2007/08, 2008/09). Microsatellite markers located near important QTLs were carefully chosen in accordance with existing literature data to validate marker trait associations (MTA). Genomic DNA was extracted using the CTAB method and PCR products were separated by capillary electrophoresis. The population structure was assigned based on molecular data in Structure v. 2.0 software, while association analysis was done by the Tassel program using the Q matrix. Nine significant associations were stable in all years investigated and eight MTA were detected to be significant in two growing seasons. Microsatellite markers which showed significant associations and stability in different seasons can be useful and suitable for marker assisted selection (MAS) in Serbian wheat breeding programs.

Keywords: agronomical traits; microsatellite markers; QTLs; Tassel; Triticum aestivum

Published: March 31, 2013  Show citation

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BRBAKLIC L, TRKULJA D, KONDIC-SPIKA A, TRESKIC S, KOBILJSKI B. Detection of QTLs for important agronomical traits in hexaploid wheat using association analysis. Czech J. Genet. Plant Breed. 2013;49(1):1-8. doi: 10.17221/64/2012-CJGPB.
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    References

    1. Anderson J.A., Churchill G.A., Autrque J.E., Tanksley S.D., Sorrells M.E. (1993): Optimizing parental selection for selection for genetic maps. Genome, 36: 181-186. Go to original source... Go to PubMed...
    2. Bradbury P.J., Zhang Z., Kroon D.E., Casstevens T.M., Ramdoss Y., Buckler E.S. (2007): Tassel: software for association mapping of complex traits in diverse samples. Bioinformatics, 23: 2633-2635. Go to original source... Go to PubMed...
    3. Breseghello F., Sorrells M.E. (2006): Association analysis as a strategy for improvement of quantitative traits in plants. Crop Science, 46: 1323-1330. Go to original source...
    4. Chaturvedi B.K., Gupta R.R. (1995): Selection parameters for some grain and quality attributes in spring wheat (Triticum aestivum L.). Agricultural Science Digest Kernal, 15: 186-190.
    5. Doyle J.J., Doyle J.L. (1990): Isolation of plant DNA from fresh tissue. Focus, 12: 13-15. Go to original source...
    6. Evanno G., Regnaut S., Goudet J. (2005): Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14: 2611-2620. Go to original source... Go to PubMed...
    7. Gupta P.K., Mir R.R., Mohan A., Kumar J. (2008): Wheat genomics: present status and future prospects. International Journal of Plant Genomics, 2-36. Go to original source... Go to PubMed...
    8. Grain genes: a database for Triticeae and Avena. Grain Genes: a database. Available at http://wheat.pw.usda.gov/GG2/index.shtml
    9. Huang S., Sirikhachornikit A., Su X., Faris J., Gill B., Haselkorn R., Gornicki P. (2002): Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase ot the Triticum/Aegilops complex and the evolutionary history of polyploid wheat. Proceedings of the National Academy of Sciences USA, 99: 8133-8138. Go to original source... Go to PubMed...
    10. Kirwigi F.M., Ginkel M.V., Brown-Guedira G., Gill B.S., Paulsen G.M., Fritz A.K. (2007): Markers associated with a QTL for grain yield in wheat under drought. Molecular Breeding, 20: 401-413. Go to original source...
    11. Kobiljski B., Quarrie S., Dencic S., Kirby J., Iveges M. (2002): Genetic diversity of the Novi Sad wheat core collection revealed by microsatellites. Cellular and Molecular Biology Letters, 7: 685-694.
    12. Korzun V., Röder M.S., Ganal M.W., Worland A.J., Law C.N. (1998): Genetic analysis of the dwarfing gene (Rht8) in wheat. Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 96: 1104-1109. Go to original source...
    13. Kuchel H., Williams K.J., Langridge P., Eagles H.A., Jefferies S.P. (2007): Genetic dissection of grain yield in bread wheat. I. QTL analysis. Theoretical and Applied Genetics, 115: 1029-1041. Go to original source... Go to PubMed...
    14. Kumar N., Kulwal P., Balyan H., Gupta P. (2007): QTL mapping for yield and yield contributing traits in two mapping populations of bread wheat. Molecular Breeding, 19: 163-177. Go to original source...
    15. Lin F., Xue S.L., Tian D.G., Li C.J., Cao Y., Zhang Z.Z., Zhang C.Q., Ma Z.Q. (2008): Mapping chromosomal regions affecting flowering time in a spring wheat RIL population. Euphytica, 164: 769-777. Go to original source...
    16. Liu L., Wang L., Yao J., Zheng Y., Zhao C. (2010): Association mapping of six agronomic traits on chromosome 4A of wheat (Triticum aestivum L.). Molecular Plant Breeding, 1. doi:10.5376/mpb.2010.01.0005 Go to original source...
    17. Ma J., Zhou R., Dong Y., Wang L., Wang X., Jia J. (2001): Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica, 120: 219-226. Go to original source...
    18. Maccaferri M., Sanguineti M.C., Corneti S., Ortega J.L.A., Salem M.B., Bort J., Deambrogio E., Garcia del Moral L.F., Demontis A., El-Ahmed A., Maalouf F., Machlab H., Martos V., Moragues M., Motawaj J., Nachit M., Nserallah N., Ouabbou H., Royo C., Slama A., Tuberosa R. (2008): Quantitative trait loci for grain yield and adaptaion of durum wheat (Triticum durum Desf.) across a wide range of water availability. Genetics, 178: 489-511. Go to original source... Go to PubMed...
    19. Neumann K., Kobiljski B., Dencic S., Varshney R.K., Börner A. (2011): Genome wide association mapping a case study in bread wheat (Triticum aestivum L.). Molecular Breeding, 27: 37-58. Go to original source...
    20. Paterson A.H., Damon S., Hewitt J.D., Zamir D., Rabinowitch H.D., Lincoln S.E., Lander E.S., Tanksley S.D. (1991): Mendelian factors underlying quantitative traits in tomato - comparison across species, generations, and environments. Genetics, 127: 181-197. Go to original source... Go to PubMed...
    21. Pestsova E., Röder M.S. (2002): Microsatellite analysis of wheat chromosome 2D allows the reconstruction of chromosomal inheritance in pedigrees of breeding programmes. Theoretical and Applied Genetics, 106: 84-91. Go to original source... Go to PubMed...
    22. Pritchard J.K., Stephens M., Donnelly P. (2000): Interference of population structure using multilocus genotype data. Genetics, 155: 945-959. Go to original source... Go to PubMed...
    23. Quarrie S.A., Dodig D., Pekic S., Kirby J., Kobiljski B. (2003): Prospects for marker-assisted selection of improved drought responses in wheat. Bulgarian Journal of Plant Physiology, 28: 83-95.
    24. Ravel C., Praud S., Murigneux A., Canaguier A., Sapet F., Samson D., Balfourier F., Dufour P., Chalhoub B., Brunel D., Beckert M., Charmet G. (2006): Single-nucleotide polymorphism frequency in a set of selected lines of bread wheat (Triticum aestivum L.). Genome, 49: 1131-1139. Go to original source... Go to PubMed...
    25. Rehman Arif M.A., Nagel M., Neumann K., Kobiljski B., Lohwasser U., Börner A. (2012): Genetic studies of seed longevity in hexaploid wheat using segregation and association mapping approaches. Euphytica, 186: 1-13. Go to original source...
    26. Röder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M-H., Leroz P., Ganal M.W. (1998): A microsatellite map of wheat. Genetics, 194: 2007-2023. Go to original source... Go to PubMed...
    27. Trkulja D., Kondic-Spika A., Brbaklic L., Kobiljski B. (2011): Marker-trait association analysis for heading and flowering time in wheat by single marker regression. Ratarstvo i povrtarstvo/Field and Vegetable Crops Research, 48: 113-120. Go to original source...
    28. Yu J., Pressoir G., Briggs W.H., Bi I.V., Yamasaki M., Doebley J.F., Mcmullen M.D., Gaut B.S., Nielsen D.M., Holland J.B., Kresovich S., Buckler E.S. (2006): A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. Nature Genetics, 38: 203-208. Go to original source... Go to PubMed...

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