Czech J. Genet. Plant Breed., 2012, 48(4):157-168 | DOI: 10.17221/111/2012-CJGPB

Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.)Original Paper

Daniel Mihálik1, Edita Gregová1, Petr Galuszka2, Ludmila Ohnoutková2, Tatiana Klempová3, Katarína Ondreičková1, Marcela Gubišová1, 4, Jozef Gubiš1, Ján Kraic1
1 Plant Production Research Center Piešťany, Piešťany, Slovak Republic
2 Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic
3 Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak Technical University, Bratislava, Slovak Republic
4 Department of Botany and Genetics, Faculty of Natural Sciences, Constantine The Philosopher University in Nitra, Nitra, Slovak Republic

A novel high-molecular-weight glutenin subunit encoded by the Glu-1D locus was identified in hexaploid wheat (Triticum aestivum L.) cultivar Noe and was designated as 1Dy12.3. This subunit differed in SDS-PAGE mobility from the well-known 1Dy10 and 1Dy12 subunits that are also encoded by this locus. An analysis of the gene sequences confirmed the uniqueness of 1Dy12.3 and revealed that it is most closely related to the 1Dy12 subunit. The size of the deduced protein was calculated to be 67 884 Da, which is different from the 1Dy10 and 1Dy12 subunits (67 475 Da and 68 713 Da, respectively). The 1Dy12.3 protein consists of 652 residues, with a highly conserved signal sequence and N- and C-terminal domains, although the central repetitive domain comprising motifs of hexapeptide (PGQGQQ) and nonapeptide (GYYPTSLQQ) repeats was less conserved. The 1Dy12.3 subunit demonstrates fewer QHPEQG hexapeptide motifs and exhibits an increased number of methionine residues in comparison to the other characterised high-molecular-weight glutenin subunits. The 1Dy12.3 subunit was cloned and expressed in Escherichia coli and was detected with a prolamin-specific antibody. The size of the detected immunocomplex corresponded to the native 1Dy12.3 protein isolated from grains. The existence and characterisation of this novel high-molecular-weight glutenin subunit increases the diversity of the glutenins encoded by the Glu-1D locus.

Keywords: 1Dy12.3; Glu-1Dy locus; high-molecular-weight glutenin subunit; Triticum aestivum L.

Published: December 31, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Mihálik D, Gregová E, Galuszka P, Ohnoutková L, Klempová T, Ondreičková K, et al.. Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.). Czech J. Genet. Plant Breed. 2012;48(4):157-168. doi: 10.17221/111/2012-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Anderson O.D., Greene F.C., Yip R.E., Halford N.G., Shewry P.R., Malpica-Romero J.M. (1989): Nucleotide sequences of the two high-molecular-weight glutenin genes from D-genome of hexaploid bread wheat, Triticum aestivum L. cv. Cheyenne. Nucleic Acids Research, 17: 461-462. Go to original source... Go to PubMed...
    2. Anderson O.D., Kuhl J.C., Tam A. (1996): Construction and expression of a synthetic wheat storage protein gene. Gene, 174: 51-58. Go to original source... Go to PubMed...
    3. Beccari J. (1745): Wheat. De Bononiensi Scientiarum et Artium Instituto atque Academia Commentarii, II. Part I., Typographia Laelii a Vulpe, Bologna, 122-127.
    4. Dotlačil L., Hermuth J., Stehno Z., Dvořáček V., Bradová J., Leišová L. (2010): How can wheat landraces contribute to present breeding? Czech Journal of Genetics and Plant Breeding, 46 (Special Issue): S70-S74. Go to original source...
    5. Dvorak J., Luo M.C., Yang Z., Zhang H.B. (1998): The structure of the Aegilops tauschii gene pool and the evolution of hexaploid wheat. Theoretical and Applied Genetics, 97: 657-670. Go to original source...
    6. Fang J., Liu Y., Zhang C., Yang G., Li K., Shewry P.R., He G. (2009): Cloning and molecular characterization of a novel x-type glutenin gene Glu-Dt1 from Aegilops tauschii. Molecular Biology Reporter, 36: 1195-1202. Go to original source... Go to PubMed...
    7. Feldman M., Lupton F.G.H., Miller T.E. (1995): Wheats. In: Smartt J., Simmonds N.W. (eds): Evolution of Crop Plants. 2 nd Ed. Longman Scientific, London, 184-192.
    8. Giles R.J., Brown T.A. (2006): Glu-Dy allele variations in Aegilops tauschii and Triticum aestivum: implications for the origins of hexaploid wheats. Theoretical and Applied Genetics, 112: 1563-1572. Go to original source... Go to PubMed...
    9. Gregová E., Hermuth J., Kraic J., Dotlačil L. (2004): Protein heterogeneity in European wheat landraces and obsolete cultivars: Additional information. Genetic Resources and Crop Evolution, 51: 569-575. Go to original source...
    10. Gianibelli M.C., Solomon R.G. (2003): A novel y-type high-Mr glutenin subunit (12.4t) present in Triticum tauschii. Journal of Cereal Sciences, 37: 253-256. Go to original source...
    11. Guo X., Guo J., Li X., Yang X., Li L. (2010): Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits. Genetic Resources and Crop Evolution, 57: 1217-1225. Go to original source...
    12. Halford N.G., Forde J., Anderson O.D., Greene F.C., Shewry P.R. (1987): The nucleotide and deduced amino acid sequence of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosome 1A and 1D. Theoretical and Applied Genetics, 75: 117-126. Go to original source...
    13. Jiang Q.T., Wei Y.M., Yan Z.H., Zheng Y.L. (2006): Isolation and sequence analysis of HMW glutenin subunit 1Dy10.1 ecoding gene from Xinjiang wheat (Triticum petropavlovskyi Udacz. et Migusch). Agricultural Science in China, 5: 101-105. Go to original source...
    14. Juhász A., Larroque O.R., Tamás L., Hsam S.L.K., Zeller F.J., Békés F., Bedő Z. (2003): Bánkuti 1201 - an old Hungarian wheat variety with special storage protein composition. Theoretical and Applied Genetics, 107: 697-704. Go to original source... Go to PubMed...
    15. Kihara H. (1944): Discovery of the DD-analyser, one of the ancestors of Triticum vulgare. Agriculture and Horticulture, 19: 13-14.
    16. Levanony H., Rubin R., Altschuler Y., Galili G. (1992): Evidence for a novel route of wheat storage proteins to vacuoles. Journal of Cell Biology, 119: 1117-1128. Go to original source... Go to PubMed...
    17. Liu B., Xu CH., Zhao N., Qi B., Kimatu J.N., Pang J., Han F. (2009): Rapid genomic changes in polyploid wheat and related species: implications for genome evolution and genetic improvement. Journal of Genetics and Genomics, 36: 519-528. Go to original source... Go to PubMed...
    18. Lu C.M., Lu B.R. (2005): Molecular characterization of the HMW glutenin genes Dtx1.5+Dty10 from Aegilops tauschii and their PCR-mediated recombinants. Molecular Breeding, 15: 247-255. Go to original source...
    19. Mackie A.M., Sharp P.J., Lagudah E.S. (1996): The nucleotide and derived amino acid sequence of a HMW glutenin gene from Triticum tauschii and comparison with those from the D genome of bread wheat. Journal of Cereal Sciences, 24: 73-78. Go to original source...
    20. McFadden E.S., Sears E.R. (1946a): The origin of Triticum spelta and its free-threshing hexaploid relatives. Journal of Heredity, 37: 81-89. Go to original source... Go to PubMed...
    21. McFadden E.S., Sears E.R. (1946b): The origin of Triticum spelta and its free-threshing hexaploid relatives. Journal of Heredity, 37: 107-116. Go to original source...
    22. Mihálik D., Šliková S., Konôpková Ľ., Gregová E., Kraic J. (2010): The selection of high molecular weight glutenin Dy-subunits for hexaploid wheat (Triticum aestivum L.) by DNA marker. Agriculture, 56: 59-63.
    23. Osborne T.B. (1907): The Proteins of the Wheat Kernel. Publications of the Carnegie Institution Washington, Vol. 84. Judd and Detweiler, Washington.
    24. Osborne T.B. (1909): The Vegetable Proteins. Longmans, Green and Co., London, xiii + 125. Go to original source...
    25. Osborne T.B. (1924): The Vegetable Proteins. 2 nd Ed. Longmans, Green and Co., London, xiii + 154.
    26. Payne P.I., Corfield K.G., Blackman J.A. (1979): Identification of high-molecular weight subunit glutenin whose presence correlates with breadmaking quality in wheats of related pedigree. Theoretical and Applied Genetics, 55: 153-159. Go to original source... Go to PubMed...
    27. Payne P.I., Nightengale M.A., Krattiger A.F., Holt L.M. (1987): The relationship between HMW glutenin subunit composition and breadmaking quality of British grown wheat varietes. Journal of the Science in Food and Agriculture, 40: 51-65. Go to original source...
    28. Schaefer L., Bábelová A., Kiss E., Hausser H.J., Baliova M., Krzyzankova M., Marsche G., Young M.F., Mihálik D., Götte M., Malle E., Schaefer R.M., Gröne H.J. (2005): The matrix component biglycan is proinflamatory and signals through toll-like receptors 4 and 2 in macrophages. Journal of Clinical Investigation, 115: 2223-2233. Go to original source... Go to PubMed...
    29. Shewry P.R., Halford N.G. (2002): Cereal seed storage proteins: structure, properties and role in grain utilization. Journal of Experimental Botany, 53: 947-958. Go to original source... Go to PubMed...
    30. Shewry P.R., Tatham A.S. (1990): The prolamin storage proteins in cereal seeds: structure and evolution. Biochemistry Journal, 267: 1-12. Go to original source... Go to PubMed...
    31. Shewry P.R., Halford N.G., Tatham A.S. (1992): The high molecular weight subunits of wheat glutenin. Journal of Cereal Sciences, 15: 105-120. Go to original source...
    32. Shewry P.R., Tatham A.S., Barro F., Barcelo P., Lazzeri P. (1995): Biotechnology of breadmaking - unravelling and manipulating the multi-protein gluten complex. Biotechnology, 13: 1185-1190. Go to original source... Go to PubMed...
    33. Shewry P.R., Popineau Y., Lafiandra D., Belton P. (2001): Wheat glutenin subunits and dough elasticity. Trend in Food Sciences and Technology, 11: 433-441. Go to original source...
    34. Thompson R.D., Bartels D., Harberd N.P. (1985): Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Research, 13: 6833-6846. Go to original source... Go to PubMed...
    35. Thompson J.D., Higgins D.G., Gibson T.J. (1994): Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 13: 6833-6846. Go to original source... Go to PubMed...
    36. Tilley K.A. (1997): Detection of O-glycosidically linked mannose within the structure of a highly purified glutenin subunit isolated from Chinese Spring wheat. Cereal Chemistry, 74: 371-374. Go to original source...
    37. Wan Y., Liu K., Wang D., Shewry P.R. (2000): Highmolecular-weight glutenin subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by thw Dx alleles of common wheat. Theoretical and Applied Genetics, 101: 879-884. Go to original source...
    38. Wrigley C.W. (1992): Identification of cereal varieties by gel electrophoresis of the grain proteins. In: Linskens H.-F., Jackson J.F. (eds): Seed Analysis. Springer-Verlag, Berlin, Heidelberg, 17-41. Go to original source...
    39. Xu S.S., Khan K., Klindworth D.L., Nygard G. (2010): Evaluation and characterization of high-molecular weight 1D glutenin subunits from Aegilops tauschii in synthetic hexaploid wheats. Journal of Cereal Sciences, 52: 333-336. Go to original source...
    40. Xu L.-L., Li W., Wei Y.-M., Zheng Y.-L. (2009): Genetic diversity of HMW glutenin subunits in diploid, tetraploid and hexaploid Triticum species. Genetic Resources and Crop Evolution, 56: 377-391. Go to original source...
    41. Yan Y., Hsam S.L.K., Yu J., Jiang Y. Zeller F.J. (2003): Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS-PAGE), acid polyacrylamide gel (A-PAGE) and capillary electrophoresis. Euphytica, 130: 377-385. Go to original source...
    42. Yan Y., Zheng J., Xiao Y., Yu J., Hu Y., Cai M., Li Y., Hsam S.L.K., Zeller F.J. (2004): Identification and molecular characterization of a novel y-type Glu-Dt1 glutenin gene of Aegilops tauschii. Theoretical and Applied Genetics, 108: 1349-1358. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content