Czech J. Genet. Plant Breed., 2011, 47(1):17-27 | DOI: 10.17221/92/2010-CJGPB

Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stressOriginal Paper

Sara Tale Ahmad, Raheem Haddad
Agricultural Biotechnology Department, Imam Khomeini International University, Qazvin, Iran

The effect of silicon (Si) was investigated on the major antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD), relative water content (RWC), chlorophyll and soluble protein contents, proline (Pro) and glycine betaine (GB) accumulation in three different growth stages (2nd, 4th leaf and tillering stages) of wheat (Triticum aestivum L.) plants under drought stress. The experiment was performed in a completely randomized design for three treatments including control, drought and Si-drought (2mM silicate sodium/kg) with three replications in a greenhouse. The results indicated that Si partially offset the negative impacts of drought stress increasing the tolerance of wheat by rising Pro and GB accumulation and soluble protein content. Compared with the plants treated with drought, applied Si significantly enhanced the activities of SOD, CAT, APX and POD. In contrast, drought stress caused a considerable decrease in RWC, chlorophyll and soluble protein contents. This Si effect was time-dependent and became stronger in the tillering stage. The results of the present experiment coincided with the conclusion that Si alleviates water deficit of wheat by preventing the oxidative membrane damage and may be associated with plant osmotic adjustment.

Keywords: drought; osmotic adjustment; oxidative stress; silicon; wheat

Published: March 31, 2011  Show citation

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Tale Ahmad S, Haddad R. Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress. Czech J. Genet. Plant Breed. 2011;47(1):17-27. doi: 10.17221/92/2010-CJGPB.
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    References

    1. Aebi H.E. (1984): Catalase in vitro. Methods in Enzymology, 105: 121-126. Go to original source... Go to PubMed...
    2. Al-Aghabary K., Zhu Z., Shi Q. (2004): Influence of silicon supply on chlorophyll content, chlorophyll fluorescence and antioxidative enzyme activities in tomato plants under salt stress. Journal of Plant Nutrition, 27: 2101-2115. Go to original source...
    3. Arnon D.I. (1949): Copper enzymes in isolated chloroplast. Poly-phenoloxidase in Beta vulgaris. Journal of Plant Physiology, 24: 1-15. Go to original source... Go to PubMed...
    4. Arrora A., Sairam R.K., Srivastava G.C. (2002): Oxidative stress and antioxidant system in plants. Journal of Plant Physiology, 82: 1227-1237.
    5. Bates L.S., Waldren R.P., Teare I.D. (1973): Rapid determination of free Pro for water stress studies. Journal of Plant Soil, 39: 205-217. Go to original source...
    6. Beauchamp C., Fridovich F. (1971): Superoxide dismutase: cadmium assay and an assay applicable to acryl amide gels. Analytical Biochemistry, 44: 276-287. Go to original source... Go to PubMed...
    7. Bradford M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    8. Carlos A.C.C., Adriano L.P., Leandro B.L., Rogerio P.S., Giuseppina P.P.L. (2009): Effects of silicon and drought stress on tuber yield and leaf biochemical characteristics in potato. Crop Science, 49: 949-954. Go to original source...
    9. Chance B., Maehly A. (1955): Assay of catalase and peroxidase. Methods in Enzymology, 2: 764-817. Go to original source...
    10. Epstein E. (1999): Silicon. Plant Physiology, 50: 641- 664. Go to original source... Go to PubMed...
    11. Gao X., Zou G.H., Wang L., Zhang F. (2006): Silicon decrease transpiration rate and conductance from stomata of maize plants. Plant Nutrition, 29: 1637-1647. Go to original source...
    12. Genard H., Saos J.L.E., Hillard J., Tremolieres A., Boucaud J. (1991): Effect of salinity on lipid composition, glycine betaine content and photosynthetic activity in chloroplasts of Suaeda maritima. Plant Physiology and Biochemistry, 29: 421-427.
    13. Gong H.Z., Chen K., Wang S., Zhang C. (2005): Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Science, 169: 313-321. Go to original source...
    14. Grieve C.M., Grattan S.R. (1983): Rapid assay for determination of water soluble quaternary ammonium compounds. Plant and Soil, 70: 303-307. Go to original source...
    15. Hart M.A., Tyson H., Bloomberg B. (1971): Measurement of activity of peroxidase isoenzymes in flax (Linum usitatissimum). Canadian Journal of Botany, 49: 2129-2137. Go to original source...
    16. Hattori T., Inanaga S., Tanimoto E., Lux A., Luxova M., Sugimoto Y. (2003): Silicon-induced changes in viscoelastic properties of sorghum root cell walls. Plant Cell Physiology, 44: 743-749. Go to original source... Go to PubMed...
    17. Hattori T., Inanaga S., Araki H., An P., Morita S., Luxova M., Lux A. (2005): Application of silicon enhanced drought tolerance in sorghum bicolor. Physiologia Plantarum, 123: 459-466. Go to original source...
    18. Hossain M.T., Mori R., Soga K., Wakabayashi K., Kamisak S., Fujii S., Yamamoto R., Hoson T. (2002): Growth promotion and an increase in cell wall extensibility by silicon in rice and some other Poaceae seedlings. Journal of Plant Research, 115: 23-27. Go to original source... Go to PubMed...
    19. Jung S. (2004): Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. Plant Science, 166: 459-466. Go to original source...
    20. Kaufman P.B., Takeoka Y., Carlson T.J., Bigelow W.C., Jones Ones J.D., Moore P.H., Ghosheh N.S. (1979): Studies on silica deposition in sugarcane (Saccharum spp.) using scanning electron microscopy, energy dispersive X-ray analysis, neutron activation analysis and light microscopy. Phytomorphology, 29:185-193.
    21. Laemmli U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685. Go to original source... Go to PubMed...
    22. Li Q.F., Ma C.C., Shang Q.L. (2007): Effects of silicon on photosynthesis and antioxidative enzymes of maize under drought stress. Chinese Journal of Applied Ecology, 18: 531-536.
    23. Liang Y.C. (1998). Effects of silicon on leaf ultrastructure, chlorophyll content and photosynthetic activity in barley under salt stress. Pedosphere, 8: 289-296.
    24. Liang Y., Chen Q., Zhang W., Ding R. (2003): Exogenous silicon increases antioxidant enzyme activity and reduces lipid peroxidation in root of salt-stressed barley (Hordeum vulgare L.). Plant Physiology, 160: 1157-1167. Go to original source... Go to PubMed...
    25. Lobato A.K.S., Coimbra G.K., Neto M.A.M., Costa R.C.L., Santos F.B.G., Oliveira C.F., Luz L.M., Barreto A.G.T., Pereira B.W.F., Alves G.A.R., Monterio B.S., Marochio C.A. (2009): Protective action of silicon on water relation and photosynthetic pigments in pepper plants induced to water deficit. Research Journal of Biological Sciences, 4: 617-623.
    26. Lux A., Luxova M., Morita S., Abe J., Inanaga S. (1999): Endodermal silicification in developing seminal roots of lowland and upland cultivars of rice (Oryza sativa L.). Canadian Journal of Botany, 77: 955-960. Go to original source...
    27. Ma J.F. (2004): Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses. Soil Science and Plant Nutrition, 50: 11-18. Go to original source...
    28. Ma J.F., Takahashi E. (2002): Soil, Fertilizer and Plant Silicon Research in Japan. Elsevier Science, Amsterdam. Go to original source...
    29. Ma J.F., Miyake Y, Takahashi E. (2001): Silicon as a beneficial element for crop plants. In: Datnoff L.E., Snyder G.H., Korndorfer G.H. (eds): Silicon in Agriculture. Studies in Plant Science No. 8, Elsevier Science, Amsterdam, 17-39. Go to original source...
    30. Maiti R.K., Ramaiah K.V., Bisen S.S., V.L. (1984): A comparative study of the haustorial development of Striga asiatica (L.) Kuntze on sorghum cultivars. Annals of Botany, 54: 447-457. Go to original source...
    31. Matichenkov V.V., Bocharnikova E.A., Ammosova J.M. (2001): The influence of silicon fertilizers on the plants and soils. Agrochemistry, 12: 30-37.
    32. McCord J.M. (2000): The evolution of free radicals and oxidative stress. American Journal of Medicine, 108: 652-659. Go to original source... Go to PubMed...
    33. Mittler R. (2002): Oxidative stress antioxidants and stress tolerance. Trends in Plant Science, 7: 405-410. Go to original source... Go to PubMed...
    34. Nakano Y., Asada K. (1987): Purification of ascorbate peroxidase in spinach chloroplast: in inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiology, 28: 131-140.
    35. Noctor G., Veljvoic-Jovanovic S., Foyer C.H. (2000): Peroxide processing in photosynthesis: antioxidant coupling and redox signalling. Philosophical Transactions of the Royal Society of London, 355: 1465-1475. Go to original source... Go to PubMed...
    36. Ranganathan S., Suvarchala V., Rajesh Y.B.R.D., Prasad M.S., Padmakumari A.P., Voleti S.R. (2006): Effect of silicon sources on its deposition, chlorophyll content, and disease and pest resistance in rice. Biologia Plantarum, 50: 713-716. Go to original source...
    37. Rao M.V., Paliyath G., Ormrod D.P. (1996): Ultraviolet-B and ozone-induced of protein biochemical change in antioxidant enzymes of Arabidopsis thaliana. Plant Physiology, 110: 125-136. Go to original source... Go to PubMed...
    38. Richmond K.E., Sussman M. (2003): Got silicon? The non-essential beneficial plant nutrient. Current Opinion in Plant Biology, 6: 268-272. Go to original source... Go to PubMed...
    39. Robertson E.F., Dannelly H.K., Malloy P.J., Reeves H.C. (1987): Rapid isoelectric focusing in a vertical polyacrylamide minigel system. Analytical Biochemistry, 167: 290-294. Go to original source... Go to PubMed...
    40. Rybicki E., Purves M. (2003): SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE). Deptartment of Microbiology, University of Cape Town.
    41. Sacala E. (2009): Role of silicon in plant resistance to water stress. Journal of Elementology, 14: 619-630.
    42. Schonfeld M.A., Johnson R.C., Carver B.F., Mornhinweg D.W. (1988): Water relations in winter wheat as drought resistance indicator. Crop Science, 28: 526-531. Go to original source...
    43. Smirnoff N. (1993): The role of active oxygen in the responses to water deficit and desiccation. New Phytologist, 125: 27-58. Go to original source... Go to PubMed...
    44. Sudhakar A., Lakshmi S., Giridarakumar A. (2001): Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science, 161: 613-619. Go to original source...
    45. Yong Y., Tai S., Bao X. (2007): Effects of silicon on photosynthesis and antioxidative enzymes of maize under drought stress. Plant Science, 18: 531-536.
    46. Zhu Z., Wei G., Li J., Qian Q., Yu J. (2004): Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Science, 167: 527-533. Go to original source...

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