Czech J. Genet. Plant Breed., 2009, 45(4):160-168 | DOI: 10.17221/72/2008-CJGPB
Ampelographic and molecular diversity among grapevine (Vitis spp.) cultivarsOriginal Paper
- 1 Department of Horticulture, Faculty of Agriculture, University of Selcuk, Konya, Turkey
- 2 Department of Horticulture, Faculty of Agriculture, University of Cukurova, Adana, Turkey
This study presents the ampelographic and molecular characterization of 44 grapevine cultivars. Ampelographic data were obtained during two vegetation periods using the latest version of the descriptors. Based on the mean values transformed by the method indicated in IBPGR publications, a dendrogram was constructed. ISSR analysis was also employed to characterize the genotypes at the DNA level. Twenty primers, selected on the basis of their discriminating potential, generated a total of 157 bands, of which 140 were polymorphic. The dendrograms constructed by the two approaches were largely similar in both the clustering position and divergence of varietal groups. The least distance was observed between Yuvarlak Cekirdeksiz and Superior Seedless. The clustering position of cultivars throughout the dendrograms was basically related to the genetic distances and main uses, as well as to geographic origins.
Keywords: ampelography; genetic distance; ISSR; Vitis spp.
Published: December 31, 2009 Show citation
References
- Agaoglu Y.s., Celik H., Gökcay E. (1990): Brief ampelographic characters of indigenous grapevine cultivars subjected to clonal selection in Turkey. 5th Int. Symp. on Grape Breeding. September 12-16, 1989, St. Martin Pfalz, Vitis Special Issue, 532-537.
- Alleweldt G., Dettweiler E. (1986): Ampelographic studies to characterize grapevine varieties. Vignevini, 13: 56-59.
- Almadanim M.C., Baleiras-Couto M.M., Pereira H.S., Carneiro L.C., Fevereiro P., Eiras-Dias J.E., Morais-Cecilio L., Viegas W., Veloso M.M. (2007): Genetic diversity for the grapevine (Vitis vinifera L.) cultivars most utilized for wine production in Portugal. Vitis, 46: 116-119.
- Anonymous (1983): Grape Descriptors. International Board for Plant Genetic Resources, Rome.
- Anonymous (1997): Descriptors for Grapevine (Vitis spp.). International Plant Genetic Resources Institute, Rome.
- Botstein D., White R.L., Skolnick M., Davis R.W. (1980): Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32: 314-331.
- Bourquin J.C., Otten L., Walter B. (1993): Restriction fragment length polymorphism and molecular taxonomy in Vitis vinifera. Theoretical and Applied Genetics, 87: 157-162.
Go to original source...
Go to PubMed... - Bowers J.E., Dangi G.S., Vignami R., Meredith C.P. (1996): Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome, 39: 628-633.
Go to original source...
Go to PubMed... - Casasoli M., Mattioni C., Cherubini M., Villani F. (2001): Genetic linkage map of European chestnut (Castenea sativa Mill.) based on RAPD, ISSR and isozyme markers. Theoretical and Applied Genetics, 102: 1190-1199.
Go to original source... - Cervera M.T., Cabezas J.A., Sanchez-Eschribano E., Cenis J.L., Martinez-Zapater J.M. (2000): Characterisation of genetic variation within table grape varieties based on AFLP markers. Vitis, 39: 109-114.
- Dhanorkar V.M., Tamhankar S.A., Patil S.G., Rao V.S. (2005): ISSR-PCR for assessment of genetic relationship among grape varieties cultivated in India. Vitis, 44: 127-131.
- Doyle J.J., Doyle J.L. (1990): Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.
Go to original source... - Ergul A., Kazan K., Aras S., Cevik V., Celik, H., Soylemezoglu G. (2006): AFLP analysis of genetic variation within the two economically important grapevine (Vitis vinifera L.) varietal groups. Genome, 49: 467-475.
Go to original source...
Go to PubMed... - Ergul A., Marasali B., Agaoglu Y.S. (2002): Molecular discrimination and identification of some Turkish grape cultivars (Vitis vinifera L.) by RAPD markers. Vitis, 41: 159-160.
- Fossati T., Labra M., Castiglione S., Failla O., Scienza A., Sala F. (2001): The use of AFLP and SSR molecular markers to decipher homonyms and synonyms in grapevine cultivars: The case of the varietal group known as 'Schiave'. Theoretical and Applied Genetics, 102: 200-205.
Go to original source... - Galet P. (1985): Precis D'ampelographie Practique. 5th Ed., Dehan, Montpellier.
- Herrera R., Cares V., Wilkinson M.J., Caligari P.D.S. (2002): Characterization of genetic variation between Vitis vinifera cultivars from central Chile using RAPD and inter simple sequence repeat markers. Euphytica, 124: 139-145.
Go to original source... - Kafkas S., Ozkan H., Ak B.E., Acar I., Atli H.S., Koyuncu S. (2006): Detecting DNA polymorphism and genetic diversity in a wide pistachio germplasm: Comparison of AFLP, ISSR and RAPD markers. Journal of the American Society for Horticultural Science, 131: 522-529.
Go to original source... - Kara Z. (1990): Determination of the ampelographic characters of grape varieties in Tokat. [Ph.D. Thesis.] Ankara.
- Martin J.P., Santiago J.L., Pinto-Carnide O., Leal F., Martinez M.C., Ortiz, J.M. (2006): Determination of relationship among autochthonous grapevine varieties (Vitis vinifera L.) in the Northwest of the Iberian Peninsula by using microsatellite markers. Genetic Resources and Crop Evolution, 53: 1255-1261.
Go to original source... - Moreno S., Martin J.P., Ortiz J.M. (1998): Intersimple sequence repeats PCR for characterization of closely related grapevine germplasm. Euphytica, 101: 117-125.
Go to original source... - Oraman M.N., Agaoglu Y.S. (1969): Some characteristics of Turkey's viticulture and the composition of its districts in viticulture. Ankara University Agriculture Faculty Yearbook, Ankara.
- Ortiz J.M., Martin J.P., Borrego J., Chavez J., Rodriguez I., Munoz G., Cabello F. (2004): Molecular and morphological characterization of a Vitis gene bank for the establishment of a base collection. Genetic Resources and Crop Evolution, 51: 403-409.
Go to original source... - Prevost A., Wilkinson M.J. (1999): A new system of comprising PCR primers applied to ISSR fingerprinting of potato accessions. Theoretical and Applied Genetics, 98: 107-112.
Go to original source... - Rholf F. (2004): NTSYSpc Version 2.1, Numerical Taxonomy and Multivariate Analysis System. Department of Ecology and Evolution, State University, New York.
- Sabir A., Kafkas S., Tangolar S., Buyukalaca S. (2008): Genetic relationship of grape cultivars by ISSR (Inter-simple sequence repeats) markers. European Journal of Horticultural Sciences, 73: 84-88.
- Santiago J.L., Boso S., Martinez M.C., PintoCarnide O., Ortiz J. M. (2005): Ampelographic comparison of grape cultivars (Vitis vinifera L.) grown in Northwestern Spain and Northern Portugal. American Journal of Enology and Viticulture, 56: 287-290.
Go to original source... - Sefc K.M., Regner F., Turetschek E., Glösl E., Steinkellner H. (1999): Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome, 42: 1-7.
Go to original source... - Thomas M.R., Matsumoto S., Cain P., Scott N.S. (1993): Repetitive DNA of grapevine: Classes present and sequences suitable for cultivar identification. Theoretical and Applied Genetics, 86: 173-180.
Go to original source...
Go to PubMed... - Weeden J., Reisch B., Martens M. (1988): Genetic analysis of isozyme polymorphism in grape. Journal of the American Society for Horticultural Science, 113: 735-769.
Go to original source... - Winkler A.J., Cook J.A., Kliewer W.M., Lider L.A. (1974): General Viticulture. University of California Press, Berkeley.
Go to original source... - Ye G.N., Soylemezoglu G., Weeden N.F., Reisch B.I. (1998): Analysis of the relationship between grapevine cultivars, sports and clones via DNA fingerprinting. Vitis, 37: 33-38.
- Zietkiewicz E., Rafalski A., Labuda D. (1994): Genome fingerprinting by simple sequence repeat (FSR)anchored polymerase chain reaction amplification. Genomics, 20: 176-183.
Go to original source...
Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.

