Czech J. Genet. Plant Breed., 2005, 41(10):314-318 | DOI: 10.17221/6206-CJGPB

Multivariate RP-HPLC analysis as a tool in quality studies in durum wheat

W.J. Rogers, J.L. Robutti, S.E. Lerner, A.M. Di Martino, N.R. Ponzio, F.S. Borrás, M. Cogliatti, M.L. Seghezzo, E.R. Molfese
Facultad de Agronomía, Universidad Nacional del Centro de la Provincia de Buenos Aires, Azul, Provincia de Buenos Aires, República Argentina
Estación Experimental Agropecuaria Pergamino - INTA, Pergamino, Provincia de Buenos Aires, República Argentina
Laboratorio de Calidad Industrial de Granos, Chacra Experimental Integrada Barrow MAGyAI - INTA, Tres Arroyos, Provincia de Buenos Aires, República Argentina
Researcher of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) of Argentina

Keywords: RP-HPLC; gliadin; principal components; industrial quality; grain sulphur content; grain protein content; Farinograph tolerance; Triticum turgidum var. durum L.

Published: December 31, 2005  Show citation

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Rogers WJ, Robutti JL, Lerner SE, Di Martino AM, Ponzio NR, Borrás FS, et al.. Multivariate RP-HPLC analysis as a tool in quality studies in durum wheat. Czech J. Genet. Plant Breed. 2005;41(Special Issue):314-318. doi: 10.17221/6206-CJGPB.
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References

  1. B J.A. (1983): Separation of cereal proteins by reversed-phase high-performance liquid chromatography. Journal of Chromatography, 255: 219-238. Go to original source... Go to PubMed...
  2. B J.A. (1990): HPLC of cereal endosperm storage proteins. In: G K., R F. (eds): HPLC of Biological Macromolecules; Methods and Applications. Marcel Dekker, Inc., NY, U.S.A: 429-455.
  3. B J.A., H F.R., N T.C., S D.G., G S., S J. (1990): Statistical interpretation of data from high-performance liquid chromatography of wheat proteins. In: B W., T R. (eds): Gluten Proteins 1990. In: Proc. of the 4th Int. Workshop on Gluten Proteins, AACC, St. Paul, MN, June 27-29, 1990: 420-432.
  4. B J.A., S D.G. (1992): Electrophoresis and chromatography of wheat proteins: Available methods and procedures for statistical evaluation of the data. Journal of Chromatography, 624: 53-80. Go to original source... Go to PubMed...
  5. B T., B J.A. (1984): Reversed-phase highperformance liquid chromatography of durum wheat gliadins: relationships to durum wheat quality. Journal of Cereal Science, 2: 3-14. Go to original source...
  6. C J.M., V J.F., O J. (1990): Relationship between gluten strength and glutenin proteins in durum wheat cultivars. Plant Breeding, 104: 325-333. Go to original source...
  7. C J.M., M M.C., M C., B M.T., N T M.T., V J.F. (2000): Relationship between endosperm proteins and quality in durum wheat (Triticum turgidum L. var. durum). Options Mediterranees, 40: 463-467.
  8. D J.W., Q J.S. (1983): A modified screening test for rapid estimation of gluten strength in earlygeneration durum wheat breeding lines. Cereal Chemistry, 60: 315-318.
  9. H F.R., B J.A. (1993): Improved chromatographic separation and characterization of ethanol-soluble wheat proteins. Cereal Chemistry, 70: 506-511.
  10. P P.I., J E.A., H L.M. (1984): The association between γ-gliadin 45 and gluten strength in durum varieties: a direct causal effect or the result of genetic linkage? Journal of Cereal Science, 2: 73-81. Go to original source...
  11. P N., L D., F P., A C. (1988): Evidence for a direct causal effect of low molecular weight subunits of glutenins on gluten viscoelasticity in durum wheats. Journal of Cereal Science, 7: 211-214. Go to original source...
  12. R J.L., B F.S., F M.E., B J.A. (2000): Grouping and identification of Argentine maize races by principal component analysis of zein reversedphase HPLC data. Cereal Chemistry, 77: 91-95. Go to original source...
  13. R W.J., C M., L S.E., P N.R., R J.L., D M A.M., B F.S., S M.L., M E.R.: Densitometric SDS-PAGE and multivariate RP-HPLC analyses of N-S fertilisation and cultivar effects on grain protein composition in durum wheat. Submi ed.
  14. R W.J., P P.I., H K. (1989): The HMW glutenin subunit and gliadin compositions of German-grown wheat varieties and their relationship with bread-making quality. Plant Breeding, 103: 89-100. Go to original source...
  15. R M., C J.M. (1995): Relationships between different prolamin proteins and some quality properties in durum wheat. Plant Breeding, 114: 40-44. Go to original source...
  16. S D.G., G S., S J., B J.A., H F.R., N T. (1989): Relating chromatographic data to measurements of wheat quality: Case studies in dimension reduction. Chemometrics and Intelligent Laboratory Systems, 10: 155-167. Go to original source...
  17. Z J.C., C T.T., K C.F. (1979): A decimal code for the growth stages of cereals, with illustrations. Annals of Applied Biology, 93: 221-234. Go to original source...

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