Czech Journal of Genetics and Plant Breeding, 2025 (vol. 61), issue 3
Genome-wide identification and in silico expression analysis of CCO gene family in Citrus sinensis (orange) in response to citrus greeningOriginal Paper
Sadaf Sarwar, Muhammad Shafiq, Layba Tasawar, Iram Gull, Jannat Akram, Maha Al-Roshdi, Gabrijel Ondrasek, Muhammad Shafiq Shahid
Czech J. Genet. Plant Breed., 2025, 61(3):113-127 | DOI: 10.17221/11/2025-CJGPB 
Citrus sinensis (L.) Osbeck (sweet orange) is the most important cultivated citrus fruit in the world. However, Hanglongbing (HLB) disease, caused by Candidatus Liberibactor asiaticus (CLAs), poses a major threat to sweet orange production, by hindering colour, quality and export. Carotenoid cleavage oxygenases (CCOs), which include carotenoid cleavage dioxygenases (CCDs) and 9-cis-epoxycarotenoid dioxygenases (NCEDs), are essential for plant growth, development, and adaptation to phytohormonal, biotic, and abiotic stresses. This study identified 14 CsCCO genes in C. sinensis. Structural and conservation studies were conducted...
Study of the genetic potential of autochthonous populations of perennial ryegrass (Lolium perenne L.) for use in breeding purposesOriginal Paper
Marina Antic, Željko Lakić, Branislav Radulović, Vera Popović
Czech J. Genet. Plant Breed., 2025, 61(3):128-137 | DOI: 10.17221/21/2025-CJGPB
The testing and evaluation of native populations of perennial ryegrasses (Lolium perenne L.) collected from the Republic of Srpska, Bosnia and Herzegovina were conducted from 2020 to 2022 at experimental fields and laboratories of the Institute for Genetic Resources and the Agricultural Institute of the Republic of Srpska in Banja Luka. Six native populations (labelled G1–G6) and two cultivars, Esquire and Tivoli (labelled G7 and G8), were analysed in this study. The following traits were examined: the plant height (cm), number of generative stems per plant, inflorescence length (cm), number of spikes per inflorescence, seed mass per inflorescence...
Impact of nitric oxide on sunflower growth and drought tolerance mechanismsOriginal Paper
Аіdаnа Sugirbеkоva, Mehmet Hamurcu, Anamika Pandey, Mohd. Kamran Khan, Fevzi Elbasan, Zuhal Zeynep Avsaroglu, Ayse Humeyra Omay, Sait Gezgin
Czech J. Genet. Plant Breed., 2025, 61(3):138-150 | DOI: 10.17221/25/2025-CJGPB
Sunflower (Helianthus annuus L.), a globally significant oilseed crop, faces substantial yield losses due to drought stress, a major environmental constraint. In this study, the effects of nitric oxide (NO) to increase drought tolerance in four sunflower genotypes (resistant Irtysh, RAR 56 and sensitive Zarya, RAR 133) showing different stress responses were investigated. Conducted in a controlled hydroponic system, the experiment applied 100 µM NO under 12% polyethylene glycol (PEG)-induced drought, assessing growth, physiological, and biochemical parameters. PEG alone reduced shoot and root growth, relative water content (RWC), and ion...
Assessing genetic variability and heritability in the F4 population of mutant Bioguma and Gando Keta local Indonesian sorghum [Sorghum bicolor (L.) Moench]Original Paper
Endang Lestari, Karlina Syahruddin, Heny Herawati, Suarni Suarni, Moch Subechan, Suwarti Suwarti, Slamet Priyanto, Herniwati Herniwati, Diana Widiastuti
Czech J. Genet. Plant Breed., 2025, 61(3):151-159 | DOI: 10.17221/15/2025-CJGPB
This study examined the genetic variability, heritability, and genetic advance in the F4 generation of sorghum to enhance grain productivity. The study was conducted at Citayam Research Station, Depok Indonesia (March–September 2023), and evaluated 102 progenies of the F4 population from a Bioguma (a mutant line) × Gando Keta (a local variety) cross using an Augmented block design with four replications. The results of this experiment indicated that the F4 generation outperformed the Gando Keta grain yield components, but remained inferior to Bioguma. The yield traits were influenced by the non-additive gene action,...
Transcriptome analysis reveals differential gene expression in tomato under high-temperature stress
Yun Li, Xin Ye, Lingzeng Lv, Na Chen
Czech J. Genet. Plant Breed., 2025, 61(3):160-179 | DOI: 10.17221/45/2025-CJGPB
Tomato is a major global crop, extensively cultivated in China. However, the molecular mechanisms underlying its responses to high-temperature stress remain poorly understood. This study investigates these mechanisms by examining a heat-resistant tomato variety, Hm 2-2 (R), and a heat-sensitive variety, BY 1-2 (S), under high temperature (40 °C). Total RNA was extracted from samples taken at 0 and 24 h post-treatment, followed by RNA-sequencing (RNA-seq). Differentially expressed genes (DEGs) were screened based on the criteria of |log2 fold change| ≥ 2 and false discovery rate ≤ 0.05. Gene ontology (GO) function annotation and...
Obituary of Assoc. Prof. RNDr. Jaroslava Ovesná, CSc. (1959-2025)Editorial
Dagmar Janovská, Dagmar Cvrčková
Czech J. Genet. Plant Breed., 2025, 61(3):180 | DOI: 10.17221/59/2025-CJGPB
It is with great sadness that we announce our respected colleague and prominent scientist, Assoc. Prof. RNDr. Jaroslava Ovesná, CSc., passed away on June 17, 2025, following a lengthy battle with an illness at the age of 65 years. She served as a member of the editorial board of the Czech Journal of Genetics and Plant Breeding from 2000 to 2017. From 1984, immediately after graduating in biochemistry from the Faculty of Science of Charles University, until her passing, she worked at the Czech Agrifood Research Centre (CARC), formerly the Crop Research Institute (CRI).