Czech J. Genet. Plant Breed., 2007, 43(3):97-104 | DOI: 10.17221/2070-CJGPB

Genetic resources of barley and oat characterised by microsatellites

Leona Leišová, Ladislav Kučera, Ladislav Dotlačil
Crop Research Institute, Prague-Ruzyně, Czech Republic

Barley (Hordeum vulgare L.) and oat (Avena sativa L.) are important crop species. 1865 accessions of winter barley, 2707 accessions of spring barley and 1998 accessions of oat are maintained in RICP Gene bank. The expert core collection is used to be established as a tool for germplasm study, conservation of genetic variability and for the identification of useful genes. The main aim of this study was to evaluate genetic diversity of barley and oat genotypes within the expert core collections. Genetic variation of 176 barley accessions was analyzed using 26 microsatellite loci, covering all 6 chromosomes. 330 oat accessions were analyzed using 26 microsatellite loci that are mapped only into linkage groups. For 26 barley microsatellite loci, 328 alleles were detected. The average number of alleles per locus was 12.6. In oat, for 26 oat microsatellite loci, 353 alleles were detected. The average number of alleles per locus was 13.6. The average DI (diversity index) was 0.11 in barley and 0.09 in oat. Dendrogram and PCA (Principal Component Analysis) based on microsatellite data showed a different influence of the place of origin, age of variety and pedigree on grouping into clusters. PCA showed that the breeding process had a negative impact on the level of genetic diversity and therefore there is a necessity of barley and oat germplasm conservation.

Keywords: barley; oat; microsatellites; genetic diversity

Published: September 30, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Leišová L, Kučera L, Dotlačil L. Genetic resources of barley and oat characterised by microsatellites. Czech J. Genet. Plant Breed. 2007;43(3):97-104. doi: 10.17221/2070-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Akkaya M.S., Bhagwat A.A., Cregan P.B. (1992): Length polymorphism of simple sequence repeat DNA in soybean. Genetics, 132: 131-139. Go to original source... Go to PubMed...
    2. Bataillon T.M., David J.L., Schoen D.J. (1996): Neutral genetic markers and conservation genetics: simulated germplasm collections. Genetics, 144: 409-417. Go to original source... Go to PubMed...
    3. Bennett M.D., Smith J.B. (1976): Nuclear DNA amounts in angiosperms. Philosophical Transactions of the Royal Society of London, Series B - Biological Sciences, 274: 227-274. Go to original source... Go to PubMed...
    4. Brown A.H.D. (1995): The core collection at the crossroads. In: Hodgkin T., Brown A.H.D., van Hintum T.J.L., Morales A.E.V. (eds.): Core Collections of Plant Genetic Resources. John Wiley and Sons, Ltd., Chichester, 3-19.
    5. Devos K.M., Bryan G.J., Collins A.J., Stephenson P., Gale M.D. (1995): Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theoretical and Applied Genetics, 90: 241-252. Go to original source... Go to PubMed...
    6. Frankel O.H., Bennett E. (1970): Genetic Resources in Plants - Their Exploration and Conservation. F.A. Davis, Philadelphia.
    7. Fu Y.B., Peterson G.W., Scoles G., Rossnagel B., Schoen D.J., Richards K.W. (2003): Allelic diversity changes in 96 Canadian oat cultivars released from 1886 to 2001. Crop Science, 43: 1989-1995. Go to original source...
    8. Gini C.W. (1912): Variabilita e mutabilita. Studi Economico-Giuridici della R. Univ Cagliari, 3: 3-159.
    9. Hernández P., Laurie D.A., Martin A., Snape J.W. (2002): Utility of wheat simple sequence repeats (SSR) markers for genetic analysis of Hordeum chilense and H. tritordeum. Theoretical and Applied Genetics, 104: 735-739. Go to original source... Go to PubMed...
    10. Korzun V., Röder M.S., Worland A.J., Börner A. (1997): Intrachromosomal mapping of the genes for dwarfing (Rht12) and vernalisation response (Vrn1) Plant Breeding, 116: 227-232. Go to original source...
    11. Leišová L., Ovesná J. (2001): The use of microsatellite analysis for the identification of wheat varieties. Czech Journal of Genetics and Plant Breeding, 37: 29-33.
    12. Li C.D., Rossnagel B.G. (2000): The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars. Theoretical and Applied Genetics, 101: 1259-1268. Go to original source...
    13. Liu Z.W., Biyashev R.M., Saghai Maroof M.A. (1996): Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics, 93: 869-873. Go to original source... Go to PubMed...
    14. Medini M., Hamza S., Rebai A., Baum M. (2005): Analysis of genetic diversity in Tunisian durum wheat cultivars and related wild species by SSR and AFLP markers. Genetic Resources and Crop Evolution, 52: 21-31. Go to original source...
    15. Pal N., Snadhu J.S., Domier L.L., Kolb F.L. (2002): Development and characterization of microsatellite and RFLP-derived PCR markers in oat. Crop Science, 42: 912-918. Go to original source...
    16. Plaschke J., Ganal M.W., Röder M. S. (1995): Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theoretical and Applied Genetics, 91: 1001-1007. Go to original source... Go to PubMed...
    17. Rajhathy T., Thomas H. (1974): Cytogenetics of oats (Avena L.). Miscellaneous Publications of the Genetics Society of Canada, No. 2, Otawa.
    18. Ramsay L., Macaulay M., Ivanissevich S., MacLean K., Cardle L., Fuller J., Edwards K.J., Tuvesson S., Morgante M., Massari A., Maestri E., Marmirolli N., Sjakste T., Ganal M., Powell W., Waugh R. (2000): A simple sequence repeat-based linkage map of barley. Genetics, 156: 1997-2005. Go to original source... Go to PubMed...
    19. Roussel V., Leisova L., Exbrayat F., Stehno Z., Balfourier F. (2005): SSR allelic diversity changes in 480 European bread wheat varieties released from 1840 to 2000. Theoretical and Applied Genetics, 111: 162-170. Go to original source... Go to PubMed...
    20. Russell J., Fuller J., Young G., Thomas B., Taramino G., Macaulay M., Wagh R., Powell W. (1997): Discriminating between barley genotypes using microsatellite markers. Genome, 40: 442-450. Go to original source... Go to PubMed...
    21. Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A., Allard R.W. (1984): Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal locations, and population dynamics. Proceedings of the National Academy of Sciences of the United States of America, 81: 8014-8018. Go to original source... Go to PubMed...
    22. Struss D., Plieske J. (1998): The use of microsatellite markers for detection of genetic diversity in barley populations. Theoretical and Applied Genetics, 97: 308-315. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content